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CACNA1H, CACNA1G (AA 400-450) antibody

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AA 400-450
Human, Rat (Rattus), Mouse (Murine)
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from aa region 400-450 of human CACNA1G conjugated to blue carrier protein was used as the antigen. The antigen shares 95% identity with the corresponding sequences in rat and mouse.
Specificity Specific for CACNA1H and CACNA1G.
Purification Whole serum
Background Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the low-voltage activated (LVA) group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.
Subcellular location: Membrane, Multi-pass membrane protein. Tissue specificity (CACNA1H): Expressed in kidney, liver, heart, brain. Isoform 2 seems to be testis-specific. Tissue specificity (CACNA1G): Highly expressed in brain, in particular in the amygdala, subthalamic nuclei, cerebellum and thalamus. Moderate expression in heart, low expression in placenta, kidney and lung. Also expressed in colon and bone marrow and in tumoral cells to a lesser extent. Highly expressed in fetal brain, but also in peripheral fetal tissues as heart, kidney and lung, suggesting a developmentally regulated expression. Also known as: Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav3.2, Low-voltage-activated calcium channel alpha1 3.2 subunit. Voltage-dependent T-type calcium channel subunit alpha-1G, Voltage-gated calcium channel subunit alpha Cav3.1, Cav3.1c, NBR13.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Martin, Han, Gordon, Terry, Prabhakar, She, Xie, Hellsten, Chan, Altherr, Couronne, Aerts, Bajorek, Black, Blumer, Branscomb, Brown, Bruno, Buckingham, Callen, Campbell, Campbell, Campbell, Caoile et al.: "The sequence and analysis of duplication-rich human chromosome 16. ..." in: Nature, Vol. 432, Issue 7020, pp. 988-94, 2004 (PubMed).

Toyota, Ho, Ohe-Toyota, Baylin, Issa: "Inactivation of CACNA1G, a T-type calcium channel gene, by aberrant methylation of its 5' CpG island in human tumors." in: Cancer research, Vol. 59, Issue 18, pp. 4535-41, 1999 (PubMed).

Williams, Washburn, Hans, Urrutia, Brust, Prodanovich, Harpold, Stauderman: "Structure and functional characterization of a novel human low-voltage activated calcium channel." in: Journal of neurochemistry, Vol. 72, Issue 2, pp. 791-9, 1999 (PubMed).

Cribbs, Lee, Yang, Satin, Zhang, Daud, Barclay, Williamson, Fox, Rees, Perez-Reyes: "Cloning and characterization of alpha1H from human heart, a member of the T-type Ca2+ channel gene family." in: Circulation research, Vol. 83, Issue 1, pp. 103-9, 1998 (PubMed).