CACNA1HCACNA1G (Cav3.2, Cav3.1) (Cytoplasmic Domain) antibody

Antigen: CACNA1HCACNA1G (Cav3.2, Cav3.1)

Binding Site: Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350144

Additional Information

UniProt: O95180

Immunogen: A synthetic peptide from a cytoplasmic domain of human CACNA1H & CACNA1G (Cav3.2, Cav3.1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 95% identity with the corresponding sequences in rat and mouse.

Format: Lyophilized

Description: Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. Subcellular location: Membrane; Multi-pass membrane protein. Tissue specificity (CACNA1H): Expressed in kidney, liver, heart, brain. Isoform 2 seems to be testis-specific. Tissue specificity (CACNA1G): Highly expressed in brain, in particular in the amygdala, subthalamic nuclei, cerebellum and thalamus. Moderate expression in heart; low expression in placenta, kidney and lung. Also expressed in colon and bone marrow and in tumoral cells to a lesser extent. Highly expressed in fetal brain, but also in peripheral fetal tissues as heart, kidney and lung, suggesting a developmentally regulated expression. Also known as: Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav3.2, Low-voltage-activated calcium channel alpha1 3.2 subunit. Voltage-dependent T-type calcium channel subunit alpha-1G, Voltage-gated calcium channel subunit alpha Cav3.1, Cav3.1c, NBR13.

Specificity: Appears to be specific for CACNA1H and CACNA1G.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Cribbs, Lee, Yang et al.: "Cloning and characterization of alpha1H from human heart, a member of the T-type Ca2+ channel gene family." in: Circulation research, Vol. 83, Issue 1, pp. 103-9, 1998 (PubMed).

Williams, Washburn, Hans et al.: "Structure and functional characterization of a novel human low-voltage activated calcium channel." in: Journal of neurochemistry, Vol. 72, Issue 2, pp. 791-9, 1999 (PubMed).

Toyota, Ho, Ohe-Toyota et al.: "Inactivation of CACNA1G, a T-type calcium channel gene, by aberrant methylation of its 5' CpG island in human tumors." in: Cancer research, Vol. 59, Issue 18, pp. 4535-41, 1999 (PubMed).

Martin, Han, Gordon et al.: "The sequence and analysis of duplication-rich human chromosome 16." in: Nature, Vol. 432, Issue 7020, pp. 988-94, 2004 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).