CACNA1I (Cav3.3) (cytoplasmic domain) antibody

Antigen: CACNA1I (Cav3.3)

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350146

Additional Information

Alternative name: CACNA1I (Cav3.3) (cytoplasmic domain)

Swiss-Prot: Q9P0X4

Immunogen: A synthetic peptide from a cytoplasmic domain of human CACNA1I (Cav3.3) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Isoform alpha-1I gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. Gates in voltage ranges similar to, but higher than alpha 1G or alpha 1H. Subcellular location: Membrane; Multi-pass membrane protein. Tissue specificity: Brain specific Also known as: Voltage-dependent T-type calcium channel subunit alpha-1I, Voltage-gated calcium channel subunit alpha Cav3.3.

Specificity: Appears to be specific for CACNA1I.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Mittman, Guo, Emerick et al.: "Structure and alternative splicing of the gene encoding alpha1I, a human brain T calcium channel alpha1 subunit." in: Neuroscience letters, Vol. 269, Issue 3, pp. 121-4, 1999 (PubMed).

Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Monteil, Chemin, Leuranguer et al.: "Specific properties of T-type calcium channels generated by the human alpha 1I subunit." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16530-5, 2000 (PubMed).

Gomora, Murbartiuan, Arias et al.: "Cloning and expression of the human T-type channel Ca(v)3.3: insights into prepulse facilitation." in: Biophysical journal, Vol. 83, Issue 1, pp. 229-41, 2002 (PubMed).

Zhang, Chen, Saraf et al.: "Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity." in: The Journal of biological chemistry, Vol. 281, Issue 31, pp. 22332-41, 2006 (PubMed).