Cathelicidin Antimicrobial Peptide (cAMP) antibody

Antigen: Cathelicidin Antimicrobial Peptide (cAMP)

Synonyms: LL37, CAP18, CRAMP, HSD26, FALL39, FALL-39, CATH-2

Clonality: Polyclonal

Host: Sheep

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350186

Additional Information

Alternative name: Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39)

Swiss-Prot: P49913

Immunogen: A synthetic peptide as a part of human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39) conjugated to an immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: Cathelicidin antimicrobial protein is an antimicrobial protein found in specific granules of polymorphonuclear leukocytes. FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils. Also known as: CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26.

Specificity: Appears to be specific for Cathelicidin antimicrobial peptide.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1:100 to 1: 3000. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Sheep antibody to human Cathelicidin antimicrobial peptide: whole serum (ABIN350186), diluted 1 : 200 in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI.  The antibody selectively recognizes polymorphonuclar cells.

Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Sheep antibody to human Cathelicidin antimicrobial peptide: whole serum (ABIN350186), diluted 1 : 200 in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclar cells.

WB on human neutrophil lysate using Sheep antibody to human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): whole serum (ABIN350186) at a dilution of 1 : 100. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Publications: Agerberth, Gunne, Odeberg et al.: "FALL-39, a putative human peptide antibiotic, is cysteine-free and expressed in bone marrow and testis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 1, pp. 195-9, 1995 (PubMed).

Cowland, Johnsen, Borregaard: "hCAP-18, a cathelin/pro-bactenecin-like protein of human neutrophil specific granules." in: FEBS letters, Vol. 368, Issue 1, pp. 173-6, 1995 (PubMed).

Larrick, Hirata, Balint et al.: "Human CAP18: a novel antimicrobial lipopolysaccharide-binding protein." in: Infection and immunity, Vol. 63, Issue 4, pp. 1291-7, 1995 (PubMed).

Gudmundsson, Agerberth, Odeberg et al.: "The human gene FALL39 and processing of the cathelin precursor to the antibacterial peptide LL-37 in granulocytes." in: European journal of biochemistry / FEBS, Vol. 238, Issue 2, pp. 325-32, 1996 (PubMed).

Larrick, Lee, Ma et al.: "Structural, functional analysis and localization of the human CAP18 gene." in: FEBS letters, Vol. 398, Issue 1, pp. 74-80, 1997 (PubMed).

Bals, Lang, Weiner et al.: "Rhesus monkey (Macaca mulatta) mucosal antimicrobial peptides are close homologues of human molecules." in: Clinical and diagnostic laboratory immunology, Vol. 8, Issue 2, pp. 370-5, 2001 (PubMed).