Insulin-Like Growth Factor 2 Receptor (IGF2R) (internal) antibody

Antigen: Insulin-Like Growth Factor 2 Receptor (IGF2R)

Synonyms: MPR1, MPRI, CD222, CIMPR, M6P-R, CI-MPR, Mpr300, AI661837, M6P/IGF2R, mpri, cd222, cimpr, m6p-r, IGF2R

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350194

Additional Information

Alternative name: Cation independent mannose 6 phosphate receptor (internal region)

Swiss-Prot: Q07113

Immunogen: A synthetic peptide from the internal region of mouse Cation independent mannose 6 phosphate receptor (Insulin-like growth factor 2 receptor) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in rat and shares 95% identity with human's.

Format: Lyophilized

Description: FUNCTION: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. SUBUNIT: Binds GGA1, GGA2 and GGA3. SUBCELLULAR LOCATION: Lysosome membrane; Single-pass type I membrane protein. Also known as: CI Man-6-P receptor, CI-MPR, M6PR, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, IGF-II receptor, M6P/IGF2 receptor, M6P/IGF2R, 300 kDa mannose 6-phosphate receptor, MPR 300, CD222 antigen, IGF2R, MPRI.

Specificity: Appears to be specific for MPRI.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: MacDonald, Pfeffer, Coussens et al.: "A single receptor binds both insulin-like growth factor II and mannose-6-phosphate." in: Science (New York, N.Y.), Vol. 239, Issue 4844, pp. 1134-7, 1988 (PubMed).

Szebenyi, Rotwein: "The mouse insulin-like growth factor II/cation-independent mannose 6-phosphate (IGF-II/MPR) receptor gene: molecular cloning and genomic organization." in: Genomics, Vol. 19, Issue 1, pp. 120-9, 1994 (PubMed).

Stoeger, Kubicka, Liu et al.: "Maternal-specific methylation of the imprinted mouse Igf2r locus identifies the expressed locus as carrying the imprinting signal." in: Cell, Vol. 73, Issue 1, pp. 61-71, 1993 (PubMed).

Cantin, Yi, Lu et al.: "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." in: Journal of proteome research, Vol. 7, Issue 3, pp. 1346-51, 2008 (PubMed).

Dephoure, Zhou, Villuen et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).