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N-Acetylglucosamine-1-Phosphotransferase, gamma Subunit (GNPTG) (Internal Region) antibody

Details for Product No. ABIN350194, Supplier: Log in to see
Antigen
  • 6430527N14Rik
  • A830081F19
  • AU067667
  • AU067744
  • Mdcp1
  • Tce7
Epitope
Internal Region
5
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
5
4
2
1
Host
Rabbit
4
2
2
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
8
6
2
1
1
1
Supplier
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Immunogen A synthetic peptide from the internal region of human Cation independent mannose 6 phosphate receptor (Insulin-like growth factor 2 receptor) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for MPRI.
Purification Whole serum
Alternative Name Mannose 6 Phosphate Receptor (GNPTG Antibody Abstract)
Background Function: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2.
Subunit: Binds GGA1, GGA2 and GGA3.
Subcellular location: Lysosome membrane, Single-pass type I membrane protein. Also known as: CI Man-6-P receptor, CI-MPR, M6PR, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, IGF-II receptor, M6P/IGF2 receptor, M6P/IGF2R, 300 kDa mannose 6-phosphate receptor, MPR 300, CD222 antigen, IGF2R, MPRI.
Application Notes A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Cantin, Yi, Lu, Park, Xu, Lee, Yates: "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." in: Journal of proteome research, Vol. 7, Issue 3, pp. 1346-51, 2008 (PubMed).

Dephoure, Zhou, Villén, Beausoleil, Bakalarski, Elledge, Gygi: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).

Szebenyi, Rotwein: "The mouse insulin-like growth factor II/cation-independent mannose 6-phosphate (IGF-II/MPR) receptor gene: molecular cloning and genomic organization." in: Genomics, Vol. 19, Issue 1, pp. 120-9, 1994 (PubMed).

Stoeger, Kubicka, Liu, Kafri, Razin, Cedar, Barlow: "Maternal-specific methylation of the imprinted mouse Igf2r locus identifies the expressed locus as carrying the imprinting signal." in: Cell, Vol. 73, Issue 1, pp. 61-71, 1993 (PubMed).

MacDonald, Pfeffer, Coussens, Tepper, Brocklebank, Mole, Anderson, Chen, Czech, Ullrich: "A single receptor binds both insulin-like growth factor II and mannose-6-phosphate." in: Science (New York, N.Y.), Vol. 239, Issue 4844, pp. 1134-7, 1988 (PubMed).