You are viewing an incomplete version of our website. Please click to reload the website as full version.

c-FOS antibody (C-Fos) (AA 300-380)

Details for Product anti-C-Fos Antibody No. ABIN350205, Supplier: Log in to see
Antigen
  • AP-1
  • c-fos
  • C-FOS
  • cFOS
  • cFos
  • D12Rfj1
  • fos
  • p55
Epitope
AA 300-380
39
23
22
20
19
18
16
13
11
9
6
6
6
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus), Mouse (Murine)
350
182
180
36
35
22
11
5
2
2
1
Host
Sheep
256
80
11
11
1
Clonality
Polyclonal
Conjugate
This c-FOS antibody is un-conjugated
8
7
7
4
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
274
129
100
39
37
35
34
22
19
13
8
7
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen A synthetic peptide from aa region 300-380 of human c Fos protein conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in rat and mouse.
Specificity Specific for cFos.
Purification Whole serum
Alternative Name c Fos
Background Function: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
Subunit: Heterodimer. Interacts with DSIPI, this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB
Subcellular location: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. Also known as: Proto-oncogene protein cFOS.
Application Notes Use at a dilution of 1 : 300 to 1 : 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Vasilescu, Zweitzig, Denis, Smith, Ethier, Haines, Figeys: "The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells." in: Journal of proteome research, Vol. 6, Issue 1, pp. 298-305, 2007

Heilig, Eckenberg, Petit, Fonknechten, Da Silva, Cattolico, Levy, Barbe, de Berardinis, Ureta-Vidal, Pelletier, Vico, Anthouard, Rowen, Madan, Qin, Sun, Du, Pepin, Artiguenave, Robert, Cruaud, Brüls et al.: "The DNA sequence and analysis of human chromosome 14. ..." in: Nature, Vol. 421, Issue 6923, pp. 601-7, 2003

Glover, Harrison: "Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA." in: Nature, Vol. 373, Issue 6511, pp. 257-61, 1995

Roux, Verrier, Klein, Niccolino, Marty, Alexandre, Piechaczyk: "Retrovirus-mediated gene transfer of a human c-fos cDNA into mouse bone marrow stromal cells." in: Oncogene, Vol. 6, Issue 11, pp. 2155-60, 1991

Hai, Liu, Coukos, Green: "Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers." in: Genes & development, Vol. 3, Issue 12B, pp. 2083-90, 1990

Nakabeppu, Nathans: "The basic region of Fos mediates specific DNA binding." in: The EMBO journal, Vol. 8, Issue 12, pp. 3833-41, 1990

van Straaten, Müller, Curran, Van Beveren, Verma: "Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 11, pp. 3183-7, 1983