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c-FOS antibody (C-Fos) (AA 300-380)

Details for Product anti-C-Fos Antibody No. ABIN350206, Supplier: Log in to see
Antigen
  • AP-1
  • c-fos
  • C-FOS
  • cFOS
  • cFos
  • D12Rfj1
  • fos
  • p55
Epitope
AA 300-380
39
23
22
19
19
18
16
13
11
8
6
6
6
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
354
185
184
36
35
22
11
5
3
2
1
1
Host
Sheep
259
83
12
10
1
Clonality
Polyclonal
Conjugate
This c-FOS antibody is un-conjugated
8
7
7
4
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
280
131
105
42
40
37
34
22
20
13
8
8
3
1
1
1
Supplier
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Immunogen A synthetic peptide from aa region 300-380 of human c Fos protein conjugated to an immunogenic carrier protein has been used as the antigen.
Isotype IgG
Specificity Specific for cFos.
Alternative Name c Fos
Background Function: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
Subunit: Heterodimer. Interacts with DSIPI, this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB
Subcellular location: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily SIMILARITY: Contains 1 bZIP domain Also known as: Proto-oncogene protein cFOS.
Application Notes Use at a concentration of 10-50 µg/ml.
The optimal working concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Vasilescu, Zweitzig, Denis, Smith, Ethier, Haines, Figeys: "The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells." in: Journal of proteome research, Vol. 6, Issue 1, pp. 298-305, 2007 (PubMed).

Heilig, Eckenberg, Petit, Fonknechten, Da Silva, Cattolico, Levy, Barbe, de Berardinis, Ureta-Vidal, Pelletier, Vico, Anthouard, Rowen, Madan, Qin, Sun, Du, Pepin, Artiguenave, Robert, Cruaud, Brüls et al.: "The DNA sequence and analysis of human chromosome 14. ..." in: Nature, Vol. 421, Issue 6923, pp. 601-7, 2003 (PubMed).

Glover, Harrison: "Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA." in: Nature, Vol. 373, Issue 6511, pp. 257-61, 1995 (PubMed).

Roux, Verrier, Klein, Niccolino, Marty, Alexandre, Piechaczyk: "Retrovirus-mediated gene transfer of a human c-fos cDNA into mouse bone marrow stromal cells." in: Oncogene, Vol. 6, Issue 11, pp. 2155-60, 1991 (PubMed).

Hai, Liu, Coukos, Green: "Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers." in: Genes & development, Vol. 3, Issue 12B, pp. 2083-90, 1990 (PubMed).

Nakabeppu, Nathans: "The basic region of Fos mediates specific DNA binding." in: The EMBO journal, Vol. 8, Issue 12, pp. 3833-41, 1990 (PubMed).

van Straaten, Müller, Curran, Van Beveren, Verma: "Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 11, pp. 3183-7, 1983 (PubMed).