Choline O-Acetyltransferase (CHAT) antibody

Antigen: Choline O-Acetyltransferase (CHAT)

Synonyms: CMS1A, CMS1A2, B230380D24Rik, CT23399, Cat, ChAT, cha, dChAT, l(3)91Cc, DmelCG12345, CG12345, CHAT, ChoAcTase, Ci-ChAT

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350212

Additional Information

Alternative name: ChAT (choline O-acetyltransferase)

Swiss-Prot: P28329

Immunogen: A synthetic peptide from human ChAT (choline O-acetyltransferase) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase  catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. Also known as: CHOACTase, choline acetylase, choline O-acetyltransferase, CMS1A, CMS1A2.

Specificity: Appears to be specific for ChAT.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Oda, Nakanishi, Deguchi: "A complementary DNA for human choline acetyltransferase induces two forms of enzyme with different molecular weights in cultured cells." in: Brain research. Molecular brain research, Vol. 16, Issue 3-4, pp. 287-94, 1993 (PubMed).

Lorenzi, Trinidad, Zhang et al.: "Two mRNAs are transcribed from the human gene for choline acetyltransferase." in: DNA and cell biology, Vol. 11, Issue 8, pp. 593-603, 1992 (PubMed).

Cervini, Rocchi, DiDonato et al.: "Isolation and sub-chromosomal localization of a DNA fragment of the human choline acetyltransferase gene." in: Neuroscience letters, Vol. 132, Issue 2, pp. 191-4, 1992 (PubMed).

Kim, Rylett, Shilton: "Substrate binding and catalytic mechanism of human choline acetyltransferase." in: Biochemistry, Vol. 45, Issue 49, pp. 14621-31, 2006 (PubMed).