Chloride Channel 1, Skeletal Muscle (CLCN1) (N-Term) antibody

Antigen: Chloride Channel 1, Skeletal Muscle (CLCN1)

Synonyms: CLCN1, CLC1, MGC138361, MGC142055, adr, mto, Clc1, Clc-1, SMCC1, nmf355, myotonia, MGC124486, SMCC

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350218

Additional Information

Alternative name: Chloride channel protein 1 (CLCN1) (n-terminal region)

Swiss-Prot: P35523

Immunogen: A synthetic peptide from the n-terminal region of human Chloride channel protein 1 (CLCN1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels. Subcellular location: Membrane, Multi-pass membrane protein. Tissue specificity: Predominantly expressed in skeletal muscles. Also known as: Skeletal muscle Chloride channel protein 1, ClC-1, CLCN1, CLC1.

Specificity: Appears to be specific for CLCN1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Koch, Steinmeyer, Lorenz et al.: "The skeletal muscle chloride channel in dominant and recessive human myotonia." in: Science (New York, N.Y.), Vol. 257, Issue 5071, pp. 797-800, 1992 (PubMed).

Lehmann-Horn, Mailaender, Heine et al.: "Myotonia levior is a chloride channel disorder." in: Human molecular genetics, Vol. 4, Issue 8, pp. 1397-402, 1995 (PubMed).

Lorenz, Meyer-Kleine, Steinmeyer et al.: "Genomic organization of the human muscle chloride channel CIC-1 and analysis of novel mutations leading to Becker-type myotonia." in: Human molecular genetics, Vol. 3, Issue 6, pp. 941-6, 1994 (PubMed).

Steinmeyer, Lorenz, Pusch et al.: "Multimeric structure of ClC-1 chloride channel revealed by mutations in dominant myotonia congenita (Thomsen)." in: The EMBO journal, Vol. 13, Issue 4, pp. 737-43, 1994 (PubMed).

Sjoeblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).