Chloride Channel 3 (CLCN3) (Cytoplasmic Domain) antibody

Antigen: Chloride Channel 3 (CLCN3)

Synonyms: CLC3, ClC-3, Clc3, CLCN3, clcn3, fb78c02, Clcn3, DKFZp459P0830, clc3, clc-3

Binding Site: Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350219

Additional Information

Alternative name: Chloride channel protein 3 (CLCN3)

UniProt: P51792

Immunogen: A synthetic peptide from the cytoplasmic domain of rat Chloride channel protein 3 (CLCN3) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory. Subcellular location: Membrane, Multi-pass membrane protein. Tissue specificity: Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus,olfactory cortex, andolfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of thePulmonary vessels. Expressed in bronchus and colon (at protein level). Also known as: ClC-3, CLCN3, CLC3.

Specificity: Appears to be specific for CLCN3.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Borsani, Rugarli, Taglialatela et al.: "Characterization of a human and murine gene (CLCN3) sharing similarities to voltage-gated chloride channels and to a yeast integral membrane protein." in: Genomics, Vol. 27, Issue 1, pp. 131-41, 1995 (PubMed).

Lamb, Clayton, Liu et al.: "Expression of CLCN voltage-gated chloride channel genes in human blood vessels." in: Journal of molecular and cellular cardiology, Vol. 31, Issue 3, pp. 657-66, 1999 (PubMed).

Huang, Liu, Di et al.: "Regulation of human CLC-3 channels by multifunctional Ca2+/calmodulin-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 23, pp. 20093-100, 2001 (PubMed).

Gentzsch, Cui, Mengos et al.: "The PDZ-binding chloride channel ClC-3B localizes to the Golgi and associates with cystic fibrosis transmembrane conductance regulator-interacting PDZ proteins." in: The Journal of biological chemistry, Vol. 278, Issue 8, pp. 6440-9, 2003 (PubMed).