Chloride Channel Kb (CLCNKB) (N-Term) antibody

Antigen: Chloride Channel Kb (CLCNKB)

Synonyms: CLCNKB, CLCNKA, DKFZp469N0132, CLCKB, Clck2, Clc-Ka, Clcnk1l, ClC-K2L, ClC-K2, ClC-Kb, hClC-Kb, MGC24087, MGC64141

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350222

Additional Information

Alternative name: Chloride channel protein ClC-Kb (CLCKB) (n-terminal region)

Swiss-Prot: P51802

Immunogen: A synthetic peptide from the n-terminal region of rat Chloride channel protein ClC-Kb (CLCKB) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms. Subcellular location: Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note: Recruited to the plasma membrane in the presence of BSND protein, whereas remains in the Golgi in its absence. Tissue specificity: Expressed predominantly in the kidney. Expressed in all segments of the nephron examined, including the S2 segment and the glomerulus. Also known as: Chloride channel Kb, ClC-K2, CLCKB.

Specificity: Appears to be specific for CLCKB.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Adachi, Uchida, Ito et al.: "Two isoforms of a chloride channel predominantly expressed in thick ascending limb of Henle's loop and collecting ducts of rat kidney." in: The Journal of biological chemistry, Vol. 269, Issue 26, pp. 17677-83, 1994 (PubMed).

Kieferle, Fong, Bens et al.: "Two highly homologous members of the ClC chloride channel family in both rat and human kidney." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 15, pp. 6943-7, 1994 (PubMed).

Waldegger, Jeck, Barth et al.: "Barttin increases surface expression and changes current properties of ClC-K channels." in: Pflügers Archiv : European journal of physiology, Vol. 444, Issue 3, pp. 411-8, 2002 (PubMed).

Hayama, Rai, Sasaki et al.: "Molecular mechanisms of Bartter syndrome caused by mutations in the BSND gene." in: Histochemistry and cell biology, Vol. 119, Issue 6, pp. 485-93, 2003 (PubMed).