Charged Multivesicular Body Protein 2A (CHMP2A) (N-Term) antibody

Antigen: Charged Multivesicular Body Protein 2A (CHMP2A)

Synonyms: BC2, BC-2, VPS2, CHMP2, VPS2A, bc2, bc-2, vps2, chmp2, vps2a, MGC78953, MGC132094

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350223

Additional Information

Alternative name: CHMP2A (Charged multivesicular body protein 2A)

UniProt: O43633

Immunogen: A synthetic peptide from the n-terminal of human CHMP2A (Charged multivesicular body protein 2A) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins. SUBCELLULAR LOCATION: Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. MISCELLANEOUS: Its overexpression strongly inhibits HIV-1 release. Also known as: Chromatin-modifying protein 2a, Vacuolar protein-sorting-associated protein 2-1, Vps2-1, hVps2-1, Putative breast adenocarcinoma marker BC-2, BC2, Charged multivesicular body protein 2a.

Specificity: Appears to be specific for CHMP2A.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Howard, Stauffer, Degnin et al.: "CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins." in: Journal of cell science, Vol. 114, Issue Pt 13, pp. 2395-404, 2001 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

von Schwedler, Stuchell, Mueller et al.: "The protein network of HIV budding." in: Cell, Vol. 114, Issue 6, pp. 701-13, 2003 (PubMed).

Kemmer, Podowski, Arenillas et al.: "NovelFam3000--uncharacterized human protein domains conserved across model organisms." in: BMC genomics, Vol. 7, pp. 48, 2006 (PubMed).

Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).