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CHMP2B antibody (Charged Multivesicular Body Protein 2B) (N-Term)

Details for Product anti-CHMP2B Antibody No. ABIN350224, Supplier: Log in to see
Antigen
  • 1190006E07Rik
  • ALS17
  • CHMP2.5
  • CHMP2B
  • chmp2b
  • chmp2bl
  • DMT1
  • RGD1306781
  • si:ch211-224m9.5
  • VPS2-2
  • VPS2B
  • zgc:77025
Epitope
N-Term
19
17
4
3
3
2
1
1
Reactivity
Mouse (Murine), Rat (Rattus), Human
64
24
22
Host
Rabbit
55
7
4
Clonality
Polyclonal
Conjugate
This CHMP2B antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
44
29
25
13
11
6
5
1
1
1
Supplier
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Supplier Product No.
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Immunogen A synthetic peptide from the n-terminal of human CHMP2B conjugated to blue carrier protein was used as the antigen.
Specificity Specific for CHMP2B.
Purification Whole serum
Alternative Name CHMP2B (CHMP2B Antibody Abstract)
Background Function: Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins.
Subcellular location: Cytoplasm, cytosol.
Tissue specificity: Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen. DISEASE: Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3). FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits. Also known as: Chromatin-modifying protein 2b, CHMP2.5, Vacuolar protein sorting associated protein 2-2, Vps2-2, hVps2-2, Charged multivesicular body protein 2b.
Research Area Neurology
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Nousiainen, Silljé, Sauer, Nigg, Körner: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Parkinson, Ince, Smith, Highley, Skibinski, Andersen, Morrison, Pall, Hardiman, Collinge, Shaw, Fisher, ,: "ALS phenotypes with mutations in CHMP2B (charged multivesicular body protein 2B)." in: Neurology, Vol. 67, Issue 6, pp. 1074-7, 2006 (PubMed).

Skibinski, Parkinson, Brown, Chakrabarti, Lloyd, Hummerich, Nielsen, Hodges, Spillantini, Thusgaard, Brandner, Brun, Rossor, Gade, Johannsen, Suorensen, Gydesen, Fisher, Collinge: "Mutations in the endosomal ESCRTIII-complex subunit CHMP2B in frontotemporal dementia." in: Nature genetics, Vol. 37, Issue 8, pp. 806-8, 2005 (PubMed).

Strack, Calistri, Craig, Popova, Göttlinger: "AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding." in: Cell, Vol. 114, Issue 6, pp. 689-99, 2003 (PubMed).

Lai, Chou, Chang, Liu, Lin: "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." in: Genome research, Vol. 10, Issue 5, pp. 703-13, 2000 (PubMed).