CHMP3 (Charged multivesicular body protein 3, VPS24) (N-Term) antibody

Antigen: CHMP3 (Charged multivesicular body protein 3, VPS24)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350225

Additional Information

UniProt: Q9Y3E7

Immunogen: A synthetic peptide from the n-terminal of human CHMP3 (Charged multivesicular body protein 3, Vacuolar protein sorting associated protein 24, VPS24) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. Required for sorting/trafficking of EGF receptor. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins. SUBCELLULAR LOCATION: Cytoplasm, cytosol. Membrane; Lipid-anchor. TISSUE SPECIFICITY: Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also known as: Chromatin-modifying protein 3, Vacuolar protein-sorting-associated protein 24, hVps24, Neuroendocrine differentiation factor, Charged multivesicular body protein 3, VPS24, NEDF.

Specificity: Appears to be specific for CHMP3.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on rat brain lysate using Rabbit antibody to n-terminal of CHMP3 (Charged multivesicular body protein 3, VPS24): whole serum (ABIN350225) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Wilson, Oh, Hwa et al.: "Interaction of IGF-binding protein-related protein 1 with a novel protein, neuroendocrine differentiation factor, results in neuroendocrine differentiation of prostate cancer cells." in: The Journal of clinical endocrinology and metabolism, Vol. 86, Issue 9, pp. 4504-11, 2001 (PubMed).

Strack, Calistri, Craig et al.: "AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding." in: Cell, Vol. 114, Issue 6, pp. 689-99, 2003 (PubMed).

von Schwedler, Stuchell, Mueller et al.: "The protein network of HIV budding." in: Cell, Vol. 114, Issue 6, pp. 701-13, 2003 (PubMed).

Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Dephoure, Zhou, Villén et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).