CHMP4B, C (N-Term) antibody

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Mouse (Murine), Rat (Rattus), Human
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from the n-terminal of human CHMP4B, C conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse, zebrafish and xenopus.
Specificity Specific for CHMP4B, C.
Purification Whole serum
Background Function: Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-I complex on endosomal membranes. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins, via the association of CHMP4 proteins with PDCD6IP/AIP1, a protein directly recruited by HIV-1 p6 protein that functions at sites of viral Gag assembly and budding.
Subcellular location: Cytoplasm.
Tissue specificity: CHMP4B: Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.
Tissue specificity: CHMP4C: Expressed in heart, spleen and kidney. DISEASE: Defects in CHMP4B are the cause of posterior polar cataract type 3 (CTPP3). Cataract is the most frequent cause of visual impairment and blindness worldwide. Posterior polar cataract is a distinctive opacity located at the back of the lens. Because of its proximity to the optical center of the eye, posterior polar cataract can have a marked effect on visual acuity.
Miscellaneous: Its overexpression strongly inhibits HIV-1 release. Also known as: CHMP4B: Chromatin-modifying protein 4b, Vacuolar protein-sorting-associated protein 7-2, SNF7-2, hSnf7-2, SNF7 homolog associated with Alix 1, hVps32, SHAX1, Charged multivesicular body protein 4b. CHMP4C: Chromatin modifying protein 4c, Vacuolar protein-sorting-associated protein 7-3, SNF7-3, hSnf7-3, SNF7 homolog associated with Alix 3, SHAX3, Charged multivesicular body protein 4c.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Shiels, Bennett, Knopf, Yamada, Yoshiura, Niikawa, Shim, Hanson: "CHMP4B, a novel gene for autosomal dominant cataracts linked to chromosome 20q." in: American journal of human genetics, Vol. 81, Issue 3, pp. 596-606, 2007 (PubMed).

Yorikawa, Shibata, Waguri, Hatta, Horii, Katoh, Kobayashi, Uchiyama, Maki: "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting." in: The Biochemical journal, Vol. 387, Issue Pt 1, pp. 17-26, 2005 (PubMed).

Katoh, Shibata, Suzuki, Nara, Ishidoh, Kominami, Yoshimori, Maki: "The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 39104-13, 2003 (PubMed).

Strack, Calistri, Craig, Popova, Göttlinger: "AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding." in: Cell, Vol. 114, Issue 6, pp. 689-99, 2003 (PubMed).

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).