Charged Multivesicular Body Protein 6 (CHMP6) (C-Term) antibody

Antigen: Charged Multivesicular Body Protein 6 (CHMP6)

Synonyms: 2400004G01Rik, VPS20, FLJ11749, RGD1565325, chmp6, vps20, MGC81346, MGC154281, MGC81952, MGC139458, DKFZp469B1316

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350230

Additional Information

Alternative name: CHMP6 (Charged multivesicular body protein 6) (c-terminal region)

Swiss-Prot: P0C0A3

Immunogen: A synthetic peptide from the c-terminal region of mouse CHMP6 (Charged multivesicular body protein 6, Chromatin-modifying protein 6) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 95% identity with rat's.

Format: Lyophilized

Description: FUNCTION: Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-II complex on endosomal membranes. SUBUNIT: Component of the endosomal sorting required for transport complex III (ESCRT-III), at least composed of CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3/VPS24, CHMP4A/SHAX2, CHMP4B/SHAX1, CHMP4C/SHAX3, CHMP5/SNF7DC2 and CHMP6/VPS20. In the complex, it interacts directly with CHMP4A, CHMP4B and CHMP4C. Interacts directly with SNF8, VPS25 and VPS36 components of the ESCRT-II complex. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Endosome membrane; Lipid-anchor . Note: Localizes to endosomal membranes. Also known as: Charged multivesicular body protein 6, Chromatin-modifying protein 6.

Specificity: Appears to be specific for CHMP6.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on rat brain lysate under reducing/denatured conditions using Rabbit antibody to C-terminal region of CHMP6 (Charged multivesicular body protein 6): whole serum (ABIN350230) at 1 : 1000 dilution. Absorption of the antibody with the immunising peptide completely abolishes the detected band. Note: the expected MW is 23.5 kD however, a band of ~70 kD is detected. This might be due to CHMP6 being a component of  a bigger complex.

Publications

Publications: Watahiki, Waki, Hayatsu et al.: "Libraries enriched for alternatively spliced exons reveal splicing patterns in melanocytes and melanomas." in: Nature methods, Vol. 1, Issue 3, pp. 233-9, 2005 (PubMed).