CNGA2 (Cyclic nucleotide gatedolfactory channel) (4th Cytoplasmic Loop) antibody

Antigen: CNGA2 (Cyclic nucleotide gatedolfactory channel)

Binding Site: 4th Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350234

Additional Information

UniProt: Q00195

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of rat CNGA2 (Cyclic nucleotide gated olfactory channel, CNG channel alpha 2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse and shares 85% identity with human sequence.

Format: Lyophilized

Description: Function: Odorant signal transduction is probably mediated by a G-protein coupled cascade using cAMP as second messenger. Theolfactory channel can be shown to be activated by cyclic nucleotides which leads to a depolarization ofolfactory sensory neurons. Subunit structure: Heterooligomer of OCNC1 and OCNC2 subunits. Subcellular location: Membrane; Multi-pass membrane protein. Tissue specificity:olfactory neurons. Also known as: Cyclic nucleotide-gatedolfactory channel, Cyclic nucleotide-gated cation channel 2, CNG channel alpha-2, CNG-2, CNG2, CNCA, CNCA1, CNCG2.

Specificity: Appears to be specific for CNGA2.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Dhallan, Yau, Schrader et al.: "Primary structure and functional expression of a cyclic nucleotide-activated channel from olfactory neurons." in: Nature, Vol. 347, Issue 6289, pp. 184-7, 1990 (PubMed).

Distler, Biel, Flockerzi et al.: "Expression of cyclic nucleotide-gated cation channels in non-sensory tissues and cells." in: Neuropharmacology, Vol. 33, Issue 11, pp. 1275-82, 1995 (PubMed).

Ruiz, London, Nadal-Ginard: "Cloning and characterization of an olfactory cyclic nucleotide-gated channel expressed in mouse heart." in: Journal of molecular and cellular cardiology, Vol. 28, Issue 7, pp. 1453-61, 1996 (PubMed).

Strijbos, Pratt, Khan et al.: "Molecular characterization and in situ localization of a full-length cyclic nucleotide-gated channel in rat brain." in: The European journal of neuroscience, Vol. 11, Issue 12, pp. 4463-7, 2000 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).