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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Contactin 1 (CNTN1) (N-Term) antibody
|Synonyms||F3, GP135, CNTN, F3cam, AW495098, CNTN1, MGC179417, F3/F11, cntn-A, F11, DKFZp459P1030|
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Quantity||100 µl (Variants)|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||Contactin-1 (Contactin 1, CNTN1)|
|Immunogen||A synthetic peptide as a part of mouse contactin-1 (F3cam, contactin 1, CNTN1) conjugated to an immunogenic carrier protein was used as the immunogen.|
|Description||FUNCTION:Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. SUBUNIT: Monomer. Interacts with CNTNAP1 in cis form. Binds to the carbonic-anhydrase like domain of protein-tyrosine phosphatase zeta. Interacts with NOTCH1 and TNR. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. MISCELLANEOUS: F3 shares with L1, N-CAM, MAG, and other cell adhesion molecules from nervous tissue the L2/HNK-1 carbohydrate epitope. Also known as: Neural cell surface protein F3, F3cam,Contactin 1, CNTN1.|
|Specificity||Appears to be specific for contactin-1.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Gennarini, Cibelli, Rougon et al.: "The mouse neuronal cell surface protein F3: a phosphatidylinositol-anchored member of the immunoglobulin superfamily related to chicken contactin." in: The Journal of cell biology, Vol. 109, Issue 2, pp. 775-88, 1989 (PubMed).
Pesheva, Gennarini, Goridis et al.: "The F3/11 cell adhesion molecule mediates the repulsion of neurons by the extracellular matrix glycoprotein J1-160/180." in: Neuron, Vol. 10, Issue 1, pp. 69-82, 1993 (PubMed).
Boyle, Berglund, Murai et al.: "Contactin orchestrates assembly of the septate-like junctions at the paranode in myelinated peripheral nerve." in: Neuron, Vol. 30, Issue 2, pp. 385-97, 2001 (PubMed).
Hu, Ang, Karsak et al.: "F3/contactin acts as a functional ligand for Notch during oligodendrocyte maturation." in: Cell, Vol. 115, Issue 2, pp. 163-75, 2003 (PubMed).
Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).
|Hosts||Rabbit (31), Goat (1)|
|Reactivities||Human (28), Rat (Rattus) (20), Mouse (Murine) (19), Cow (Bovine) (18), Dog (Canine) (18), Horse (Equine) (18), Pig (Porcine) (18)|
|Applications||Western Blotting (WB) (17), Immunofluorescence (IF) (15), ELISA (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (4), Center (2), Internal Region (1)|