Contactin 1 (CNTN1) (N-Term) antibody

Antigen: Contactin 1 (CNTN1)

Synonyms: F3, GP135, CNTN, F3cam, AW495098, CNTN1, MGC179417, F3/F11, cntn-A, F11, DKFZp459P1030

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350242

Additional Information

Alternative name: Contactin-1 (Contactin 1, CNTN1)

UniProt: P12960

Immunogen: A synthetic peptide as a part of mouse contactin-1 (F3cam, contactin 1, CNTN1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION:Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. SUBUNIT: Monomer. Interacts with CNTNAP1 in cis form. Binds to the carbonic-anhydrase like domain of protein-tyrosine phosphatase zeta. Interacts with NOTCH1 and TNR. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. MISCELLANEOUS: F3 shares with L1, N-CAM, MAG, and other cell adhesion molecules from nervous tissue the L2/HNK-1 carbohydrate epitope. Also known as: Neural cell surface protein F3, F3cam,Contactin 1, CNTN1.

Specificity: Appears to be specific for contactin-1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Gennarini, Cibelli, Rougon et al.: "The mouse neuronal cell surface protein F3: a phosphatidylinositol-anchored member of the immunoglobulin superfamily related to chicken contactin." in: The Journal of cell biology, Vol. 109, Issue 2, pp. 775-88, 1989 (PubMed).

Pesheva, Gennarini, Goridis et al.: "The F3/11 cell adhesion molecule mediates the repulsion of neurons by the extracellular matrix glycoprotein J1-160/180." in: Neuron, Vol. 10, Issue 1, pp. 69-82, 1993 (PubMed).

Boyle, Berglund, Murai et al.: "Contactin orchestrates assembly of the septate-like junctions at the paranode in myelinated peripheral nerve." in: Neuron, Vol. 30, Issue 2, pp. 385-97, 2001 (PubMed).

Hu, Ang, Karsak et al.: "F3/contactin acts as a functional ligand for Notch during oligodendrocyte maturation." in: Cell, Vol. 115, Issue 2, pp. 163-75, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).