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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Contactin 1 (CNTN1) (N-Term) antibody
|Synonyms||F3, GP135, CNTN, F3cam, AW495098, CNTN1, MGC179417, F3/F11, cntn-A, F11, DKFZp459P1030|
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|6 references available|
|Quantity||100 µl (Variants)|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||contactin-1 (contactin 1, CNTN1)|
|Immunogen||A synthetic peptide from human contactin-1 (F3cam, contactin 1, CNTN1) conjugated to an immunogenic carrier protein was used as the immunogen.|
|Description||FUNCTION:Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. SUBUNIT: Monomer. Interacts with CNTNAP1 in cis form. Binds to the carbonic-anhydrase like domain of protein-tyrosine phosphatase zeta. Interacts with NOTCH1 and TNR. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. MISCELLANEOUS: F3 shares with L1, N-CAM, MAG, and other cell adhesion molecules from nervous tissue the L2/HNK-1 carbohydrate epitope. TISSUE SPECIFICITY: Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle. Also known as: Neural cell surface protein F3, F3cam,Contactin 1, CNTN1, Glycoprotein gp135.|
|Specificity||Appears to be specific for contactin-1.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Berglund, Stigbrand, Carlsson: "Isolation and characterization of a membrane glycoprotein from human brain with sequence similarities to cell adhesion proteins from chicken and mouse." in: European journal of biochemistry / FEBS, Vol. 197, Issue 2, pp. 549-54, 1991 (PubMed).
Berglund, Ranscht: "Molecular cloning and in situ localization of the human contactin gene (CNTN1) on chromosome 12q11-q12." in: Genomics, Vol. 21, Issue 3, pp. 571-82, 1994 (PubMed).
Reid, Bronson, Young et al.: "Identification and characterization of the human cell adhesion molecule contactin." in: Brain research. Molecular brain research, Vol. 21, Issue 1-2, pp. 1-8, 1994 (PubMed).
Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).
Liu, Qian, Gritsenko et al.: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005 (PubMed).
Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).
|Hosts||Rabbit (31), Goat (1)|
|Reactivities||Human (28), Mouse (Murine) (20), Rat (Rattus) (20), Cow (Bovine) (18), Dog (Canine) (18), Horse (Equine) (18), Pig (Porcine) (18)|
|Applications||Western Blotting (WB) (17), Immunofluorescence (IF) (15), ELISA (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (4), Center (2), Internal Region (1)|