Contactin 1 (CNTN1) (N-Term) antibody

Antigen: Contactin 1 (CNTN1)

Synonyms: F3, GP135, CNTN, F3cam, AW495098, CNTN1, MGC179417, F3/F11, cntn-A, F11, DKFZp459P1030

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350243

Additional Information

Alternative name: contactin-1 (contactin 1, CNTN1)

UniProt: Q12860

Immunogen: A synthetic peptide from human contactin-1 (F3cam, contactin 1, CNTN1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION:Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. SUBUNIT: Monomer. Interacts with CNTNAP1 in cis form. Binds to the carbonic-anhydrase like domain of protein-tyrosine phosphatase zeta. Interacts with NOTCH1 and TNR. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. MISCELLANEOUS: F3 shares with L1, N-CAM, MAG, and other cell adhesion molecules from nervous tissue the L2/HNK-1 carbohydrate epitope. TISSUE SPECIFICITY: Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle. Also known as: Neural cell surface protein F3, F3cam,Contactin 1, CNTN1, Glycoprotein gp135.

Specificity: Appears to be specific for contactin-1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Berglund, Stigbrand, Carlsson: "Isolation and characterization of a membrane glycoprotein from human brain with sequence similarities to cell adhesion proteins from chicken and mouse." in: European journal of biochemistry / FEBS, Vol. 197, Issue 2, pp. 549-54, 1991 (PubMed).

Berglund, Ranscht: "Molecular cloning and in situ localization of the human contactin gene (CNTN1) on chromosome 12q11-q12." in: Genomics, Vol. 21, Issue 3, pp. 571-82, 1994 (PubMed).

Reid, Bronson, Young et al.: "Identification and characterization of the human cell adhesion molecule contactin." in: Brain research. Molecular brain research, Vol. 21, Issue 1-2, pp. 1-8, 1994 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Liu, Qian, Gritsenko et al.: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).