Contactin 4 (CNTN4) (N-Term) antibody

Antigen: Contactin 4 (CNTN4)

Synonyms: Axcam, Big-2, AXCAM, BIG-2, CNTN4A, MGC33615, 9630050B05, FAR-1, MGC84491, axcam, big-2, cntn4a

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350249

Additional Information

Alternative name: Contactin-4 (Contactin 4, BIG-2, CNTN4A)

UniProt: Q8IWV2

Immunogen: A synthetic peptide as a part of human contactin-4 (AXCAM, contactin 4, BIG-2, SCA16, CNTN4A) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. FUNCTION:Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Secreted. TISSUE SPECIFICITY: Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex. Also known as: Brain-derived immunoglobulin superfamily protein 2, AXCAM,Contactin 4, BIG-2, SCA16, CNTN4A, MGC33615.

Specificity: Appears to be specific for contactin-4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Kamei, Takeda, Teramoto et al.: "Human NB-2 of the contactin subgroup molecules: chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members." in: Genomics, Vol. 69, Issue 1, pp. 113-9, 2000 (PubMed).

Zeng, Zhang, Xu et al.: "A novel splice variant of the cell adhesion molecule contactin 4 ( CNTN4) is mainly expressed in human brain." in: Journal of human genetics, Vol. 47, Issue 9, pp. 497-9, 2002 (PubMed).

Hansford, Smith, Haber et al.: "Cloning and characterization of the human neural cell adhesion molecule, CNTN4 (alias BIG-2)." in: Cytogenetic and genome research, Vol. 101, Issue 1, pp. 17-23, 2003 (PubMed).

Fernandez, Morgan, Davis et al.: "Disruption of contactin 4 (CNTN4) results in developmental delay and other features of 3p deletion syndrome." in: American journal of human genetics, Vol. 74, Issue 6, pp. 1286-93, 2004 (PubMed).

Liu, Qian, Gritsenko et al.: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Miura, Shibata, Furuya et al.: "The contactin 4 gene locus at 3p26 is a candidate gene of SCA16." in: Neurology, Vol. 67, Issue 7, pp. 1236-41, 2006 (PubMed).