Archain 1 (ARCN1) (internal part) antibody

Antigen: Archain 1 (ARCN1)

Synonyms: cb334, id:ibd3027, MGC68969, copd, COPD, Tb08.5H5.420, nur17, MGC27546, MGC102316, 4632432M07Rik, MGC94158

Epitope: internal part

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350256

Additional Information

Alternative name: COPD (Archain, ARCN1, Coatomer subunit delta, Delta-coat protein, Delta-COP) (internal part)

Swiss-Prot: P48444

Immunogen: A synthetic peptide from the internal part of human, rat, mouse, xenopus and bovine COPD (Archain, ARCN1, Coatomer subunit delta, Delta-coat protein, Delta-COP) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. SUBCELLULAR LOCATION: Cytoplasm.  Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Also known as: Archain, ARCN1, Coatomer subunit delta, Delta-coat protein, Delta-COP.

Specificity: Appears to be specific for COPD.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 4000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Radice, Pensotti, Jones et al.: "The human archain gene, ARCN1, has highly conserved homologs in rice and Drosophila." in: Genomics, Vol. 26, Issue 1, pp. 101-6, 1995 (PubMed).

Faulstich, Auerbach, Orci et al.: "Architecture of coatomer: molecular characterization of delta-COP and protein interactions within the complex." in: The Journal of cell biology, Vol. 135, Issue 1, pp. 53-61, 1996 (PubMed).