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Dihydropyrimidinase-Like 2 (DPYSL2) (pSer518), (pThr514) antibody

Details for Product No. ABIN350261, Supplier: Log in to see
Antigen
  • AI851130
  • CRMP-2
  • CRMP2
  • Crmp2
  • crmp2
  • CRMP 2
  • CRMP2A
  • DHPRP2
  • dhprp2
  • dpysl2
  • DRP-2
  • drp-2
  • drp2
  • DRP2
  • Musunc33
  • N2A3
  • PCRMP-2
  • PCRMP 2
  • PCRMP2
  • PfCRMP-2
  • PfCRMP 2
  • TOAD-64
  • ULIP-2
  • ULIP2
  • Ulip2
  • unc-33
  • unc33
Epitope
pThr514, pSer518
29
24
14
10
6
6
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
126
89
86
5
5
4
4
1
1
1
1
Host
Rabbit
105
16
6
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
3
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (IHC), Western Blotting (WB)
89
55
42
33
19
15
4
4
3
2
Supplier
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Immunogen A synthetic peptide from the phosphorylated sites of human T514 & S518 of CRMP2 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat, mouse and chicken.
Specificity Specific for CRMP2.
Purification Whole serum
Alternative Name CRMP2 (DPYSL2 Antibody Abstract)
Background Function: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Subunit: Homotetramer, and heterotetramer with CRMP1, DPYSL3, DPYSL4 or DPYSL5. Interacts through its C-terminus with the C-terminus of CYFIP1/SRA1. Interacts with HTR4.
Subcellular location: Cytoplasm.
Tissue specificity: Ubiquitous. Also known as: Dihydropyrimidinase-related protein 2, DRP-2, Collapsin response mediator protein 2, N2A3, DPYSL2, CRMP2.
Research Area Neurology, Alzheimer's Disease
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. This antibody recognises mainly phospho T514 & S518 of CRMP-2 (but also it is quite likely to recognise the unphosphorylated form).
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Stenmark, Ogg, Flodin, Flores, Kotenyova, Nyman, Nordlund, Kursula: "The structure of human collapsin response mediator protein 2, a regulator of axonal growth." in: Journal of neurochemistry, Vol. 101, Issue 4, pp. 906-17, 2007 (PubMed).

Beausoleil, Villén, Gerber, Rush, Gygi: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Sjöblom, Jones, Wood, Parsons, Lin, Barber, Mandelker, Leary, Ptak, Silliman, Szabo, Buckhaults, Farrell, Meeh, Markowitz, Willis, Dawson, Willson, Gazdar, Hartigan, Wu, Liu, Parmigiani, Park et al.: "The consensus coding sequences of human breast and colorectal cancers. ..." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Inagaki, Chihara, Arimura, Muenager, Kawano, Matsuo, Nishimura, Amano, Kaibuchi: "CRMP-2 induces axons in cultured hippocampal neurons." in: Nature neuroscience, Vol. 4, Issue 8, pp. 781-2, 2001 (PubMed).

Gu, Hamajima, Ihara: "Neurofibrillary tangle-associated collapsin response mediator protein-2 (CRMP-2) is highly phosphorylated on Thr-509, Ser-518, and Ser-522." in: Biochemistry, Vol. 39, Issue 15, pp. 4267-75, 2000 (PubMed).

Kitamura, Takayama, Hamajima, Nakanishi, Sasaki, Endo, Takemoto, Kimura, Iwaki, Nonaka: "Characterization of the human dihydropyrimidinase-related protein 2 (DRP-2) gene." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 6, Issue 5, pp. 291-7, 2000 (PubMed).

Hamajima, Matsuda, Sakata, Tamaki, Sasaki, Nonaka: "A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution." in: Gene, Vol. 180, Issue 1-2, pp. 157-63, 1997 (PubMed).

Goshima, Nakamura, Strittmatter, Strittmatter: "Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33." in: Nature, Vol. 376, Issue 6540, pp. 509-14, 1995 (PubMed).