Dihydropyrimidinase-Like 2 (DPYSL2) (pThr514, Ser518) antibody

Antigen: Dihydropyrimidinase-Like 2 (DPYSL2)

Synonyms: drp2, crmp2, drp-2, unc33, dhprp2, unc-33, MGC68668, CRMP2, CRMP-2, DPYSL2, CRMP 2, PCRMP 2, PCRMP-2, PCRMP2, PfCRMP 2, PfCRMP-2, DRP2, DRP-2, DHPRP2, TOAD-64, DKFZp468O1328, Crmp2, Ulip2, AI851130, Musunc33, CRMP2A

Binding Site: pThr514, Ser518

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350261

Additional Information

Alternative name: CRMP-2 (Dihydropyrimidinase-related protein 2)

UniProt: Q16555

Immunogen: A synthetic peptide from the phosphorylated sites of human T514 & S518 of CRMP-2 (DPYSL2, CRMP2, Dihydropyrimidinase-related protein 2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse and chicken.

Format: Lyophilized

Description: FUNCTION: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. SUBUNIT: Homotetramer, and heterotetramer with CRMP1, DPYSL3, DPYSL4 or DPYSL5. Interacts through its C-terminus with the C-terminus of CYFIP1/SRA1. Interacts with HTR4. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Ubiquitous. Also known as: Dihydropyrimidinase-related protein 2, DRP-2, Collapsin response mediator protein 2, N2A3, DPYSL2, CRMP2.

Specificity: Appears to be specific for CRMP2.

Synonyms: drp2, crmp2, drp-2, unc33, dhprp2, unc-33, MGC68668, CRMP2, CRMP-2, DPYSL2, CRMP 2, PCRMP 2, PCRMP-2, PCRMP2, PfCRMP 2, PfCRMP-2, DRP2, DRP-2, DHPRP2, TOAD-64, DKFZp468O1328, Crmp2, Ulip2, AI851130, Musunc33, CRMP2A

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. This antibody recognises mainly phospho T514S518 of CRMP-2 (but also it is quite likely to recognise the unphosphorylated form).

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Goshima, Nakamura, Strittmatter et al.: "Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33." in: Nature, Vol. 376, Issue 6540, pp. 509-14, 1995 (PubMed).

Hamajima, Matsuda, Sakata et al.: "A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution." in: Gene, Vol. 180, Issue 1-2, pp. 157-63, 1997 (PubMed).

Kitamura, Takayama, Hamajima et al.: "Characterization of the human dihydropyrimidinase-related protein 2 (DRP-2) gene." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 6, Issue 5, pp. 291-7, 2000 (PubMed).

Gu, Hamajima, Ihara: "Neurofibrillary tangle-associated collapsin response mediator protein-2 (CRMP-2) is highly phosphorylated on Thr-509, Ser-518, and Ser-522." in: Biochemistry, Vol. 39, Issue 15, pp. 4267-75, 2000 (PubMed).

Inagaki, Chihara, Arimura et al.: "CRMP-2 induces axons in cultured hippocampal neurons." in: Nature neuroscience, Vol. 4, Issue 8, pp. 781-2, 2001 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Stenmark, Ogg, Flodin et al.: "The structure of human collapsin response mediator protein 2, a regulator of axonal growth." in: Journal of neurochemistry, Vol. 101, Issue 4, pp. 906-17, 2007 (PubMed).