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ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) antibody

Details for Product No. ABIN350270, Supplier: Log in to see
Antigen
  • apg4a
  • autl2
  • F6E13.27
  • APG4A
  • zgc:111958
  • Aut2a
  • AUTL2
  • AI627006
  • AV169859
  • Apg4a
  • Atg4al
  • Autl2
  • Atg4a
Reactivity
Human, Mouse (Murine)
67
35
21
1
1
1
Host
Rabbit
61
3
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
40
24
19
12
11
10
7
1
1
Supplier
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Immunogen A synthetic peptide from human ATG4A conjugated to an immunogenic carrier protein has been used as the antigen.
Specificity Specific for ATG4A.
Purification Whole serum
Alternative Name ATG4A (ATG4A Antibody Abstract)
Background Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified.
Function: Cysteine protease required forautophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Enzyme regulation: Inhibited by N-ethylmaleimide
Subcellular location: Cytoplasm Also known as:autophagy-related protein 4 homolog A, Autophagin-2,autophagy-related cysteine endopeptidase 2, AUT-like 2 cysteine endopeptidase, Apg4a, Autl2, AI627006, AV169859, MGC107179.
Research Area Cancer, Autophagy, Proteolysis / Ubiquitin
Pathways
Application Notes Use at a dilution of 1 : 200 to 1 : 2000.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Mariño, Uría, Puente et al.: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).