ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) antibody

Antigen: ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A)

Synonyms: APG4A, AUTL2, Apg4a, Autl2, Atg4al, AI627006, AV169859, MGC107179, Aut2a, apg4a, autl2, MGC82614, zgc:111958, F6E13.27, DKFZp459N0219

Clonality: Polyclonal

Host: Sheep

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350271

Additional Information

Alternative name: cysteine protease ATG4A (autophagin-2, Apg4a, AUTL2)

UniProt: Q8WYN0

Immunogen: A synthetic peptide as a part of human, mouse cysteine protease ATG4A (autophagin-2, Apg4a, Autl2) conjugated to an immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. FUNCTION: Cysteine protease required forautophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide SUBCELLULAR LOCATION: Cytoplasm Also known as:autophagy-related protein 4 homolog A, Autophagin-2,autophagy-related cysteine endopeptidase 2, AUT-like 2 cysteine endopeptidase, Apg4a, Autl2, AI627006, AV169859, MGC107179.

Specificity: Appears to be specific for ATG4A.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1 : 200 to 1 : 3000. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Mariño, Uría, Puente et al.: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).