ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B) antibody

Antigen: ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B)

Synonyms: Apg4b, MGC112887, ATG4B, APG4B, Aut2b2, apg4b, Atg4b, AUTL1, MGC1353

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350272

Additional Information

Alternative name: cysteine protease ATG4B (autophagin-1, APG4B, AUTL1)

UniProt: Q9Y4P1

Immunogen: A synthetic peptide from human cysteine protease ATG4B (autophagin-1, APG4B, AUTL1) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in mouse.

Format: Lyophilized

Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. FUNCTION: Cysteine protease required forautophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide SUBCELLULAR LOCATION: Cytoplasm Also known as: APG4B, AUTL1, hAPG4B, Autophagin-1, AUTL1, MGC1353, KIAA0943, cysteine protease ATG4B, hAPG4B, AUT-like 1 cysteine endopeptidase,autophagy-related protein 4 homolog B.

Specificity: Appears to be specific for ATG4B.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Images

WB on human neutrophil lysate using Rabbit antibody to human, mouse cysteine protease ATG4B (autophagin-1, APG4B, AUTL1): whole serum (ABIN350272) at a dilution of 1 : 100. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Mariño, Uría, Puente et al.: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).

Kabeya, Mizushima, Yamamoto et al.: "LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation." in: Journal of cell science, Vol. 117, Issue Pt 13, pp. 2805-12, 2004 (PubMed).

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

Kumanomidou, Mizushima, Komatsu et al.: "The crystal structure of human Atg4b, a processing and de-conjugating enzyme for autophagosome-forming modifiers." in: Journal of molecular biology, Vol. 355, Issue 4, pp. 612-8, 2005 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).