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ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B) antibody
|Synonyms||Apg4b, MGC112887, ATG4B, APG4B, Aut2b2, apg4b, Atg4b, AUTL1, MGC1353|
Human, Mouse (Murine)
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||cysteine protease ATG4B (autophagin-1, APG4B, AUTL1)|
|Immunogen||A synthetic peptide from human cysteine protease ATG4B (autophagin-1, APG4B, AUTL1) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in mouse.|
|Description||Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. FUNCTION: Cysteine protease required forautophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide SUBCELLULAR LOCATION: Cytoplasm Also known as: APG4B, AUTL1, hAPG4B, Autophagin-1, AUTL1, MGC1353, KIAA0943, cysteine protease ATG4B, hAPG4B, AUT-like 1 cysteine endopeptidase,autophagy-related protein 4 homolog B.|
|Specificity||Appears to be specific for ATG4B.|
|Application Notes||IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Mariño, Uría, Puente et al.: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).
Kabeya, Mizushima, Yamamoto et al.: "LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation." in: Journal of cell science, Vol. 117, Issue Pt 13, pp. 2805-12, 2004 (PubMed).
Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).
Kumanomidou, Mizushima, Komatsu et al.: "The crystal structure of human Atg4b, a processing and de-conjugating enzyme for autophagosome-forming modifiers." in: Journal of molecular biology, Vol. 355, Issue 4, pp. 612-8, 2005 (PubMed).
Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).
|Hosts||Rabbit (62), Mouse (1), Sheep (1)|
|Reactivities||Human (63), Mouse (Murine) (30), Rat (Rattus) (24), Cow (Bovine) (22), Dog (Canine) (21), Chicken (10), Pig (Porcine) (2), Xenopus laevis (2), Cat (Feline) (1), Zebrafish (1), Zebrafish (Danio rerio) (1)|
|Applications||Western Blotting (WB) (41), ELISA (31), Immunofluorescence (IF) (21), Immunohistochemistry (IHC) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (5), pSer309 (5), C-Term (3), AA 350-400 (1), Internal Region (1), pSer121 (1), pSer261 (1)|