ATG4B antibody (ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae))

Details for Product anti-ATG4B Antibody No. ABIN350273, Supplier: Log in to see
Antigen
  • apg4b
  • APG4B
  • AUTL1
  • 2510009N07Rik
  • AW048066
  • Apg4b
  • Atg4bl
  • Autl1
  • Aut2b2
  • autophagy related 4B, cysteine peptidase
  • autophagy-related 4b
  • cysteine protease ATG4b
  • cysteine protease ATG4B
  • ATG4 autophagy related 4 homolog B (S. cerevisiae)
  • ATG4B
  • Atg4b
  • AT3G59950
  • Tsp_08582
  • Tsp_11599
  • atg4b
Reactivity
Human, Mouse (Murine)
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Host
Sheep
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Clonality
Polyclonal
Conjugate
This ATG4B antibody is un-conjugated
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Application
Immunohistochemistry (IHC), Western Blotting (WB)
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Options
Supplier
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Immunogen A synthetic peptide from human ATG4B conjugated to an immunogenic carrier protein has been used as the antigen.
Specificity Specific for ATG4B.
Purification Whole serum
Alternative Name ATG4B (ATG4B Antibody Abstract)
Background Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed.
Function: Cysteine protease required forautophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Enzyme regulation: Inhibited by N-ethylmaleimide
Subcellular location: Cytoplasm Also known as: APG4B, AUTL1, hAPG4B, Autophagin-1, AUTL1, MGC1353, KIAA0943, cysteine protease ATG4B, hAPG4B, AUT-like 1 cysteine endopeptidase,autophagy-related protein 4 homolog B.
Research Area Cancer, Autophagy, Proteolysis / Ubiquitin
Application Notes Use at a dilution of 1 : 200 to 1 : 2000.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Kumanomidou, Mizushima, Komatsu, Suzuki, Tanida, Sou, Ueno, Kominami, Tanaka, Yamane: "The crystal structure of human Atg4b, a processing and de-conjugating enzyme for autophagosome-forming modifiers." in: Journal of molecular biology, Vol. 355, Issue 4, pp. 612-8, 2005 (PubMed).

Kabeya, Mizushima, Yamamoto, Oshitani-Okamoto, Ohsumi, Yoshimori: "LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation." in: Journal of cell science, Vol. 117, Issue Pt 13, pp. 2805-12, 2004 (PubMed).

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

Mariño, Uría, Puente, Quesada, Bordallo, López-Otín: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).

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