Dynactin 1 (DCTN1) (internal) antibody

Antigen: Dynactin 1 (DCTN1)

Synonyms: P135, HMN7B, DP-150, DAP-150, Chip150, DCTN1, MGC148413, DKFZp469C1813, DKFZp459A1630

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350279

Additional Information

Alternative name: DCTN1 (DAP-150, DP-150, Dynactin subunit 1) (internal region)

Swiss-Prot: Q14203

Immunogen: A synthetic peptide from the internal region of human DCTN1 (DAP-150, DP-150, p150-glued, p135, Dynactin subunit 1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and moue.

Format: Lyophilized

Description: FUNCTION: Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. SUBUNIT: Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein. Interacts with the C-terminus of MAPRE1, MAPRE2 and MAPRE3. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. TISSUE SPECIFICITY: Brain. DISEASE: Defects in DCTN1 are the cause of progressive lower motor neuron disease. PLMND is a progressive dominant disease that has no sensory symptoms. Also known as: DAP-150, DP-150, p150-glued,p135, Dynactin subunit 1, 150 kDa dynein-associated polypeptide.

Specificity: Appears to be specific for DCTN1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on rat brain lysate using Rabbit antibody to internal part of DCTN1 (DAP-150, DP-150, p150-glued, p135, Dynactin subunit 1): whole serum (ABIN350279) at 1 : 500 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Publications: Holzbaur, Tokito: "Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization." in: Genomics, Vol. 31, Issue 3, pp. 398-9, 1997 (PubMed).

Tokito, Howland, Lee et al.: "Functionally distinct isoforms of dynactin are expressed in human neurons." in: Molecular biology of the cell, Vol. 7, Issue 8, pp. 1167-80, 1997 (PubMed).

Collin, Nishina, Marshall et al.: "Human DCTN1: genomic structure and evaluation as a candidate for Alström syndrome." in: Genomics, Vol. 53, Issue 3, pp. 359-64, 1998 (PubMed).

Tokito, Holzbaur: "The genomic structure of DCTN1, a candidate gene for limb-girdle muscular dystrophy (LGMD2B)." in: Biochimica et biophysica acta, Vol. 1442, Issue 2-3, pp. 432-6, 1998 (PubMed).

Puls, Jonnakuty, LaMonte et al.: "Mutant dynactin in motor neuron disease." in: Nature genetics, Vol. 33, Issue 4, pp. 455-6, 2003 (PubMed).

Bu, Su: "Characterization of functional domains of human EB1 family proteins." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49721-31, 2003 (PubMed).