Doc2b (Doc2-Beta, Double C2-Like Domain-Containing Protein Beta) antibody

Antigen: Doc2b (Doc2-Beta, Double C2-Like Domain-Containing Protein Beta)

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350292

Additional Information

Swiss-Prot: P70610

Immunogen: A synthetic peptide from the n-terminal of rat Doc2b ( Doc2-beta, Double C2-like domain-containing protein beta) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse and shares 92% identity with human's.

Format: Lyophilized

Description: FUNCTION: May be involved in constitutive membrane trafficking including dynein-dependent intracellular vesicle transport. In vitro, binds calcium and phospholipids. SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. TISSUE SPECIFICITY: Widely expressed. Also known as: Double C2-like domain-containing protein beta, Doc2-beta.

Specificity: Appears to be specific for DOC2B.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Kojima, Fukuda, Aruga et al.: "Calcium-dependent phospholipid binding to the C2A domain of a ubiquitous form of double C2 protein (Doc2 beta)." in: Journal of biochemistry, Vol. 120, Issue 3, pp. 671-6, 1997 (PubMed).

Nagano, Orita, Sasaki et al.: "Interaction of Doc2 with tctex-1, a light chain of cytoplasmic dynein. Implication in dynein-dependent vesicle transport." in: The Journal of biological chemistry, Vol. 273, Issue 46, pp. 30065-8, 1998 (PubMed).