DNA-Damage Regulated Autophagy Modulator 1 (DRAM1) (internal) antibody

Antigen: DNA-Damage Regulated Autophagy Modulator 1 (DRAM1)

Synonyms: DRAM, FLJ11259, Dram, 1200002N14Rik, PRO180, TMEM77, WWFQ154, MGC54289, RP5-1180E21.1, DRAM1, dram, flj11259, MGC108229, MGC187403

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350294

Additional Information

Alternative name: DRAM (Damage-regulatedautophagy modulator) (internal region)

Swiss-Prot: Q8N682

Immunogen: A synthetic peptide from the internal part of human DRAM (Damage-regulated autophagy modulator) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen shares 85% identity with mouse's and rat's.

Format: Lyophilized

Description: This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction ofautophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. FUNCTION: Lysosomal modulator ofautophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediatedautophagy. SUBCELLULAR LOCATION: Lysosome membrane; Multi-pass membrane protein. INDUCTION: By p53/TP53 and p73/TP73. Directly activated by p53/TP53. Significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing. Also known as: Damage-regulatedautophagy modulator, FLJ11259.

Specificity: Appears to be specific for DRAM.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Images

WB on PC12 cell lysate using Rabbit antibody to DRAM (Damage-regulated autophagy modulator): whole serum (ABIN350296) at 1 : 300 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Publications: Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Crighton, Wilkinson, OPrey et al.: "DRAM, a p53-induced modulator of autophagy, is critical for apoptosis." in: Cell, Vol. 126, Issue 1, pp. 121-34, 2006 (PubMed).

Crighton, OPrey, Bell et al.: "p73 regulates DRAM-independent autophagy that does not contribute to programmed cell death." in: Cell death and differentiation, Vol. 14, Issue 6, pp. 1071-9, 2007 (PubMed).