DNA-Damage Regulated Autophagy Modulator 1 (DRAM1) (N-Term) antibody

Antigen: DNA-Damage Regulated Autophagy Modulator 1 (DRAM1)

Synonyms: DRAM, FLJ11259, Dram, 1200002N14Rik, PRO180, TMEM77, WWFQ154, MGC54289, RP5-1180E21.1, DRAM1, dram, flj11259, MGC108229, MGC187403

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350296

Additional Information

Alternative name: DRAM (Damage-regulatedautophagy modulator) (n-terminal)

Swiss-Prot: Q9DC58

Immunogen: A synthetic peptide from the n-terminal of mouse DRAM (Damage-regulated autophagy modulator) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in rat and shares 86% identity with human's.

Format: Lyophilized

Description: This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction ofautophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. FUNCTION: Lysosomal modulator ofautophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediatedautophagy. SUBCELLULAR LOCATION: Lysosome membrane; Multi-pass membrane protein. INDUCTION: By p53/TP53 and p73/TP73. Directly activated by p53/TP53. Significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing. Also known as: Damage-regulatedautophagy modulator.

Specificity: Appears to be specific for DRAM.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Images

WB on PC12 cell lysate using Rabbit antibody to n-terminal of DRAM (Damage-regulated autophagy modulator): whole serum (ABIN350296) at 1 : 300 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Publications: Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).