Dynamin 1 (Dynamin-1, B-dynamin, Dynamin, Dnm1) (C-Term) antibody

Antigen: Dynamin 1 (Dynamin-1, B-dynamin, Dynamin, Dnm1)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350298

Additional Information

UniProt: P21575

Immunogen: A synthetic peptide from the c-terminal region of rat Dynamin 1 (Dynamin-1, B-dynamin, Dynamin, Dnm1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human and mouse.

Format: Lyophilized

Description: Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis. Catalytic activity: GTP + H(2)O = GDP + phosphate. Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton. Also known as: Dynamin-1, D100, DNM, EC 3.6.5.5, B dynamin, DNM 1, DNM1, Dynamin, Dynamin BREDNM19, Dynamin1.

Specificity: Appears to be specific for Dynamin 1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Obar, Collins, Hammarback et al.: "Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins." in: Nature, Vol. 347, Issue 6290, pp. 256-61, 1990 (PubMed).

Ringstad, Nemoto, De Camilli: "The SH3p4/Sh3p8/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 16, pp. 8569-74, 1997 (PubMed).

Cousin, Robinson: "The dephosphins: dephosphorylation by calcineurin triggers synaptic vesicle endocytosis." in: Trends in neurosciences, Vol. 24, Issue 11, pp. 659-65, 2001 (PubMed).

Reubold, Eschenburg, Becker et al.: "Crystal structure of the GTPase domain of rat dynamin 1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 37, pp. 13093-8, 2005 (PubMed).

Graham, Anggono, Bache et al.: "The in vivo phosphorylation sites of rat brain dynamin I." in: The Journal of biological chemistry, Vol. 282, Issue 20, pp. 14695-707, 2007 (PubMed).