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Details for Product No. ABIN350302

EGFP/GFP/Venus antibody

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Antigen
Reactivity
»Alternatives Aequorea victoria
Host
»Alternatives Sheep
Clonality Polyclonal
Application
»Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN350302
Quantity 500 µg
Price
454.67 $   Plus shipping costs $45.00
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Availability Will be delivered in 7 to 8 Business Days
Immunogen Synthetic peptides (a pentamer) from EGFP/GFP conjugated to an immunogenic carrier protein was used as the immunogen.
Isotype IgG
Specificity Appears to be specific for EGFP/GFP.
Purity IgG
Background GFP is a  naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin’s blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Also known as: Enhanced Green Fluorescence Protein.
Application Notes IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Storage -20 °C
Yang, Moss, Phillips: "The molecular structure of green fluorescent protein." in: Nature biotechnology, Vol. 14, Issue 10, pp. 1246-51, 1998 (PubMed).

Tsien: "The green fluorescent protein." in: Annual review of biochemistry, Vol. 67, pp. 509-44, 1998 (PubMed).

Elsliger, Wachter, Hanson et al.: "Structural and spectral response of green fluorescent protein variants to changes in pH." in: Biochemistry, Vol. 38, Issue 17, pp. 5296-301, 1999 (PubMed).

Bishop, Fielding, Dyson et al.: "Systematic insertional mutagenesis of a streptomycete genome: a link between osmoadaptation and antibiotic production." in: Genome research, Vol. 14, Issue 5, pp. 893-900, 2004 (PubMed).

Alternatives for antigen "EGFP/GFP/Venus", type "Antibodies"
Hosts (2), (1), (1)
Reactivities (4)
Applications (4), (4)
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