Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (extracellular domain) antibody

Antigen: Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3)

Synonyms: EA6, EAAT1, GLAST, GLAST1, FLJ25094, Gmt1, Eaat1, GLU-T, GluT-1, MGluT1, GLAST-1, AI504299, B430115D02Rik, AF001784, CG3747, dEAAT, dEaat1, dEAAT1, Dglt-1, Dglut-1, DmelCG3747, EAAT, MGC140731, zgc:56661, zgc:77768, wu:fb98e05, wu:fd50b05, SLC1A3

Epitope: extracellular domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350309

Additional Information

Alternative name: Excitatory amino acid transporter 1 (EAAT1, GLAST, GLAST1) (extracellular domain)

Swiss-Prot: P43003

Immunogen: A synthetic peptide from extracellular domain of human Excitatory amino acid transporter 1 (EAAT1, GLAST, GLAST1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 95% identity with rat and mouse sequence.

Format: Lyophilized

Description: FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6). EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Also known as: Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, Solute carrier family 1 member 3, SLC1A3, EAAT1, GLAST, GLAST1.

Specificity: Appears to be specific for EAAT1.

Synonyms: EA6, EAAT1, GLAST, GLAST1, FLJ25094, Gmt1, Eaat1, GLU-T, GluT-1, MGluT1, GLAST-1, AI504299, B430115D02Rik, AF001784, CG3747, dEAAT, dEaat1, dEAAT1, Dglt-1, Dglut-1, DmelCG3747, EAAT, MGC140731, zgc:56661, zgc:77768, wu:fb98e05, wu:fd50b05, SLC1A3

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Arriza, Fairman, Wadiche et al.: "Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 14, Issue 9, pp. 5559-69, 1994 (PubMed).

Kawakami, Tanaka, Nakayama et al.: "Cloning and expression of a human glutamate transporter." in: Biochemical and biophysical research communications, Vol. 199, Issue 1, pp. 171-6, 1994 (PubMed).

Shashidharan, Plaitakis: "Cloning and characterization of a glutamate transporter cDNA from human cerebellum." in: Biochimica et biophysica acta, Vol. 1216, Issue 1, pp. 161-4, 1993 (PubMed).

Stoffel, Sasse, Dueker et al.: "Human high affinity, Na(+)-dependent L-glutamate/L-aspartate transporter GLAST-1 (EAAT-1): gene structure and localization to chromosome 5p11-p12." in: FEBS letters, Vol. 386, Issue 2-3, pp. 189-93, 1996 (PubMed).

Dephoure, Zhou, Villuen et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).