Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (C-Term) antibody

Antigen: Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3)

Synonyms: EA6, EAAT1, GLAST, GLAST1, FLJ25094, Gmt1, Eaat1, GLU-T, GluT-1, MGluT1, GLAST-1, AI504299, B430115D02Rik, AF001784, CG3747, dEAAT, dEaat1, dEAAT1, Dglt-1, Dglut-1, DmelCG3747, EAAT, MGC140731, zgc:56661, zgc:77768, wu:fb98e05, wu:fd50b05, SLC1A3

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350310

Additional Information

Alternative name: Excitatory amino acid transporter 1 (EAAT1, GLAST, GLAST1) (c-terminal region)

Swiss-Prot: P56564

Immunogen: A synthetic peptide from c-terminal region of mouse Excitatory amino acid transporter 1 (EAAT1, GLAST, GLAST1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat.

Format: Lyophilized

Description: FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6). EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Also known as: Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, Solute carrier family 1 member 3, SLC1A3, EAAT1, GLAST, GLAST1.

Specificity: Appears to be specific for EAAT1.

Synonyms: EA6, EAAT1, GLAST, GLAST1, FLJ25094, Gmt1, Eaat1, GLU-T, GluT-1, MGluT1, GLAST-1, AI504299, B430115D02Rik, AF001784, CG3747, dEAAT, dEaat1, dEAAT1, Dglt-1, Dglut-1, DmelCG3747, EAAT, MGC140731, zgc:56661, zgc:77768, wu:fb98e05, wu:fd50b05, SLC1A3

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Storck, Schulte, Hofmann et al.: "Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 22, pp. 10955-9, 1992 (PubMed).

Li, Niedzielski, Beisel et al.: "Identification of a glutamate/aspartate transporter in the rat cochlea." in: Hearing research, Vol. 78, Issue 2, pp. 235-42, 1995 (PubMed).

Schulte, Stoffel: "UDP galactose:ceramide galactosyltransferase and glutamate/aspartate transporter. Copurification, separation and characterization of the two glycoproteins." in: European journal of biochemistry / FEBS, Vol. 233, Issue 3, pp. 947-53, 1996 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).