Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2) (C-Term) antibody

Antigen: Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2)

Synonyms: GLT1, Eaat2, GLT-1, MGLT1, AI159670, 1700091C19Rik, 2900019G14Rik, Glt, Glt-1, EAAT, dEAAT2, DmelCG3159, CG3159, Eaat, GB16377, EAAT2, fj34b12, zgc:65897, wu:fj34b12, SLC1A2, DKFZp459A0719

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350312

Additional Information

Alternative name: Excitatory amino acid transporter 2 (EAAT2, SLC1A2) (c-terminal region)

Swiss-Prot: P31596

Immunogen: A synthetic peptide from c-terminal region of rat Excitatory amino acid transporter 2 (EAAT2, EAA2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 95% identity with human and mouse sequence.

Format: Lyophilized

Description: Mediates dihydrokainate-sensitive glutamate transport; may play a role in presynaptic terminal glutamate transport and synaptic transmission. FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. SUBUNIT: Homotrimer. Interacts with JUB. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Localized in brain and is highly enriched in the Purkinje cell layer in cerebellum, retina. Also known as: Sodium-dependent glutamate/aspartate transporter 2, GLUT-R, GLT-1, Solute carrier family 1 member 2, Slc1a2, Eaat2, Glt1.

Specificity: Appears to be specific for EAAT2.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Pines, Danbolt, Bjuoruas et al.: "Cloning and expression of a rat brain L-glutamate transporter." in: Nature, Vol. 360, Issue 6403, pp. 464-7, 1992 (PubMed).

Zhang, Pines, Kanner: "Histidine 326 is critical for the function of GLT-1, a (Na+ + K+)-coupled glutamate transporter from rat brain." in: The Journal of biological chemistry, Vol. 269, Issue 30, pp. 19573-7, 1994 (PubMed).

Kanner: "Glutamate transporters from brain. A novel neurotransmitter transporter family." in: FEBS letters, Vol. 325, Issue 1-2, pp. 95-9, 1993 (PubMed).

Pollard, McGivan: "The rat hepatoma cell line H4-II-E-C3 expresses high activities of the high-affinity glutamate transporter GLT-1A." in: FEBS letters, Vol. 484, Issue 2, pp. 74-6, 2000 (PubMed).

Marie, Billups, Bedford et al.: "The amino terminus of the glial glutamate transporter GLT-1 interacts with the LIM protein Ajuba." in: Molecular and cellular neurosciences, Vol. 19, Issue 2, pp. 152-64, 2002 (PubMed).

Rauen, Wiessner, Sullivan et al.: "A new GLT1 splice variant: cloning and immunolocalization of GLT1c in the mammalian retina and brain." in: Neurochemistry international, Vol. 45, Issue 7, pp. 1095-106, 2004 (PubMed).