Exocyst Complex Component 5 (EXOC5) (internal) antibody

Antigen: Exocyst Complex Component 5 (EXOC5)

Synonyms: SEC10, HSEC10, SEC10P, PRO1912, SEC10L1, DKFZp666H126, Sec10l1, Gm76, AI447711, AI448003, MGC80624, sec10l1, exocyst complex component sec10

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350318

Additional Information

Alternative name: Exocyst complex component 5 (SEC10, SEC10L1, EXOC5) (internal region)

Swiss-Prot: O00471

Immunogen: A synthetic peptide from the internal part of human Exocyst complex component 5 (SEC10, SEC10L1, EXOC5, Exocyst complex component Sec10) conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen is homologous with the corresponding sequence in mouse and rat.

Format: Lyophilized

Description: FUNCTION: Component of the exocyst complex involved in the docking of exocystic vesicles with fusion sites on the plasma membrane. SUBUNIT: The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Ubiquitous. Also known as: Exocyst complex component Sec10, EXOC5, SEC10, SEC10L1.

Specificity: Appears to be specific for SEC10.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IHC on rat brain using Rabbit antibody to internal part of Exocyst complex component 5 (SEC10, SEC10L1, EXOC5, Exocyst complex component Sec10): whole serum (ABIN350318) at 1 : 2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat brain using Rabbit antibody to internal part of Exocyst complex component 5 (SEC10, SEC10L1, EXOC5, Exocyst complex component Sec10): whole serum (ABIN350318) at 1 : 2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat brain using Rabbit antibody to internal part of Exocyst complex component 5 (SEC10, SEC10L1, EXOC5, Exocyst complex component Sec10): whole serum (ABIN350318) at 1 : 2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Publications

Publications: Hazuka, Hsu, Scheller: "Characterization of a cDNA encoding a subunit of the rat brain rsec6/8 complex." in: Gene, Vol. 187, Issue 1, pp. 67-73, 1997 (PubMed).

Guo, Roth, Gatti et al.: "Identification and characterization of homologues of the Exocyst component Sec10p." in: FEBS letters, Vol. 404, Issue 2-3, pp. 135-9, 1997 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).