Fibroblast Growth Factor 2 (Basic) (FGF2) (Internal Region) antibody

Antigen: Fibroblast Growth Factor 2 (Basic) (FGF2)

Synonyms: BFGF, FGFB, HBGF-2, bFGF, Fgf-2, FGF2, nudt6, fgf2, MGC52602, FGF-2, bfgf, fgfb, fgf-2, xfgf2, hbgh-2, HBGF2, LOC100227798, Fgfb

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350323

Additional Information

Alternative name: fibroblast growth factor 2 (FGF2)

UniProt: P09038

Immunogen: A synthetic peptide from the internal region of human basic FGF conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen is homologous in many other species including rat, mouse and zebrafish.

Format: Lyophilized

Description: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Also known as: BFGF, FGFB, HBGF-2, prostatropin.

Specificity: Appears to be specific for FGF2.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Watson, Anthony, Pickett et al.: "Reverse transcription with nested polymerase chain reaction shows expression of basic fibroblast growth factor transcripts in human granulosa and cumulus cells from in vitro fertilisation patients." in: Biochemical and biophysical research communications, Vol. 187, Issue 3, pp. 1227-31, 1992 (PubMed).

Zhu, Komiya, Chirino et al.: "Three-dimensional structures of acidic and basic fibroblast growth factors." in: Science (New York, N.Y.), Vol. 251, Issue 4989, pp. 90-3, 1991 (PubMed).

Ago, Kitagawa, Fujishima et al.: "Crystal structure of basic fibroblast growth factor at 1.6 A resolution." in: Journal of biochemistry, Vol. 110, Issue 3, pp. 360-3, 1992 (PubMed).

Prats, Kaghad, Prats et al.: "High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 6, pp. 1836-40, 1989 (PubMed).

Sekhon, Proskocil, Clark et al.: "Prenatal nicotine exposure increases connective tissue expression in foetal monkey pulmonary vessels." in: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology, Vol. 23, Issue 6, pp. 906-15, 2004 (PubMed).

Arkudas, Tjiawi, Bleiziffer et al.: "Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model." in: Molecular medicine (Cambridge, Mass.), Vol. 13, Issue 9-10, pp. 480-7, 2007 (PubMed).

Neuzil, Swettenham, Wang et al.: "alpha-Tocopheryl succinate inhibits angiogenesis by disrupting paracrine FGF2 signalling." in: FEBS letters, Vol. 581, Issue 24, pp. 4611-5, 2007 (PubMed).

Alexander, Watt, Sawaji et al.: "Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB." in: Arthritis and rheumatism, Vol. 56, Issue 11, pp. 3715-25, 2007 (PubMed).