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Details for Product No. ABIN350329

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody

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Antigen
Synonyms
G3PD, GAPD, Gapd, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, G3PDH, CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh, Gapdh-2, Gapdh13F, BEST:GH12586, CG12055,  ... show more
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Rat (Rattus), Human, Mouse (Murine)
Host
»Alternatives Sheep
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN350329
Quantity 250 µL
Price
454.67 $   Plus shipping costs $45.00
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Availability Will be delivered in 7 to 8 Business Days
Immunogen A synthetic peptide from c-terminal of rat GAPDH (Glyceraldehyde 3 phosphate dehydrogenase) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse and shares 90% identity with human sequence.
Specificity Appears to be specific for GAPDH.
Purity whole serum
Alternative Name GAPDH (Glyceraldehyde 3 phosphate dehydrogenase)
Background Function: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed.
Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit: Homotetramer.
Subcellular location: Cytoplasm.
Tissue specificity: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Also known as: 38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase.
UniProt P04797
Application Notes IHC, WB. A dilution of 1 : 500 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Storage -20 °C
Fort, Marty, Piechaczyk et al.: "Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family." in: Nucleic acids research, Vol. 13, Issue 5, pp. 1431-42, 1985 (PubMed).

Tso, Sun, Kao et al.: "Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene." in: Nucleic acids research, Vol. 13, Issue 7, pp. 2485-502, 1985 (PubMed).

Mohr, Stamler, Bruene: "Posttranslational modification of glyceraldehyde-3-phosphate dehydrogenase by S-nitrosylation and subsequent NADH attachment." in: The Journal of biological chemistry, Vol. 271, Issue 8, pp. 4209-14, 1996 (PubMed).

Kullberg, Nilsson, Arnason et al.: "Housekeeping genes for phylogenetic analysis of eutherian relationships." in: Molecular biology and evolution, Vol. 23, Issue 8, pp. 1493-503, 2006 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).

Alternatives for antigen "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)", type "Antibodies"
Hosts (250), (200), (37), (22), (19)
Reactivities (398), (244), (211), (108), (59), (39), (38), (36), (27), (26), (24), (20), (16), (14), (13), (11), (10), (9), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (446), (238), (140), (81), (79), (59), (50), (37), (17), (13), (7), (6), (5), (5), (4), (2), (2), (1), (1)
Conjugates (30), (24), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1)
Epitopes (81), (34), (11), (10), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
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