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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Quantity||250 µl (Variants)|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||GAPDH (Glyceraldehyde 3 phosphate dehydrogenase)|
|Immunogen||A synthetic peptide from c-terminal of rat GAPDH (Glyceraldehyde 3 phosphate dehydrogenase) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse and shares 90% identity with human sequence.|
|Description||FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Also known as: 38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase.|
|Specificity||Appears to be specific for GAPDH.|
|Synonyms||G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17|
|Application Notes||IHC, WB. A dilution of 1 : 500 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
|WB on human neutrophil lysate using Sheep antibody to c-terminal of GAPDH (Glyceraldehyde 3 phosphate dehydrogenase): whole serum (ABIN350329) at 1: 3000 dilution.|
Fort, Marty, Piechaczyk et al.: "Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family." in: Nucleic acids research, Vol. 13, Issue 5, pp. 1431-42, 1985 (PubMed).
Tso, Sun, Kao et al.: "Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene." in: Nucleic acids research, Vol. 13, Issue 7, pp. 2485-502, 1985 (PubMed).
Mohr, Stamler, Bruene: "Posttranslational modification of glyceraldehyde-3-phosphate dehydrogenase by S-nitrosylation and subsequent NADH attachment." in: The Journal of biological chemistry, Vol. 271, Issue 8, pp. 4209-14, 1996 (PubMed).
Kullberg, Nilsson, Arnason et al.: "Housekeeping genes for phylogenetic analysis of eutherian relationships." in: Molecular biology and evolution, Vol. 23, Issue 8, pp. 1493-503, 2006 (PubMed).
Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).