Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 1 (GNB1) (internal) antibody

Antigen: Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 1 (GNB1)

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350331

Additional Information

Alternative name: GBB1 (Transducin beta chain 1, GNB1, Guanine nucleotide binding protein subunit beta-1) (internal region)

Swiss-Prot: P62873

Immunogen: A synthetic peptide from the internal part of human GBB1 (Transducin beta chain 1, GNB1, Guanine nucleotide binding protein subunit beta-1) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in many species including rat, mouse, zebra fish and xenopus.

Format: Lyophilized

Description: FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in varioustransmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18. Also known as: Transducin beta chain 1, GNB1, Guanine nucleotide binding protein subunit beta-1.

Specificity: Appears to be specific for GBB1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Codina, Stengel, Woo et al.: "Beta-subunits of the human liver Gs/Gi signal-transducing proteins and those of bovine retinal rod cell transducin are identical." in: FEBS letters, Vol. 207, Issue 2, pp. 187-92, 1986 (PubMed).

Niu, Profirovic, Pan et al.: "G Protein betagamma subunits stimulate p114RhoGEF, a guanine nucleotide exchange factor for RhoA and Rac1: regulation of cell shape and reactive oxygen species production." in: Circulation research, Vol. 93, Issue 9, pp. 848-56, 2003 (PubMed).