GBB2 (N-Term) antibody

Details for Product No. ABIN350332, Supplier: Log in to see
Human, Rat (Rattus), Mouse (Murine)
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from the n-terminal region of human Transducin beta chain 2 (GBB2, GNB2, G protein subunit beta-2) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for GBB2.
Purification Whole serum
Background Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene contains a trinucleotide (CCG) repeat length polymorphism in its 5' UTR.
Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in varioustransmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Subunit: G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18. Also known as: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2, GNB2.
Application Notes A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Ballif, Carey, Sunyaev, Gygi: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).

Niu, Profirovic, Pan, Vaiskunaite, Voyno-Yasenetskaya: "G Protein betagamma subunits stimulate p114RhoGEF, a guanine nucleotide exchange factor for RhoA and Rac1: regulation of cell shape and reactive oxygen species production." in: Circulation research, Vol. 93, Issue 9, pp. 848-56, 2003 (PubMed).

Kitanaka, Wang, Kitanaka, Hembree, Uhl: "Genomic organization of the murine G protein beta subunit genes and related processed pseudogenes." in: DNA sequence : the journal of DNA sequencing and mapping, Vol. 12, Issue 5-6, pp. 345-54, 2002 (PubMed).

Wilson, Riemer, Martindale, Schnupf, Boright, Cheung, Hardy, Schwartz, Scherer, Tsui, Miller, Koop: "Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5." in: Nucleic acids research, Vol. 29, Issue 6, pp. 1352-65, 2001 (PubMed).

Arnot, Stotz, Jarvis, Zamponi: "Differential modulation of N-type 1B and P/Q-type 1A calcium channels by different G protein subunit isoforms." in: The Journal of physiology, Vol. 527 Pt 2, pp. 203-12, 2000 (PubMed).

Glöckner, Scherer, Schattevoy, Boright, Weber, Tsui, Rosenthal: "Large-scale sequencing of two regions in human chromosome 7q22: analysis of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17 genes." in: Genome research, Vol. 8, Issue 10, pp. 1060-73, 1998 (PubMed).

Williams, Schultz, Kopf: "G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development." in: Molecular reproduction and development, Vol. 44, Issue 3, pp. 315-23, 1997 (PubMed).

Gao, Gilman, Robishaw: "A second form of the beta subunit of signal-transducing G proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 17, pp. 6122-5, 1987 (PubMed).

Fong, Amatruda, Birren, Simon: "Distinct forms of the beta subunit of GTP-binding regulatory proteins identified by molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 11, pp. 3792-6, 1987 (PubMed).