Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 4 (GNB4) (internal) antibody

Antigen: Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 4 (GNB4)

Synonyms: G(beta)4, 6720453A21Rik

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350334

Additional Information

Alternative name: GBB4 (Transducin beta chain 4, GNB4, Guanine nucleotide binding protein subunit beta-4) (internal region)

Swiss-Prot: Q9HAV0

Immunogen: A synthetic peptide from an internal part of human GBB4 (Transducin beta chain 4, GNB4, Guanine nucleotide binding protein subunit beta-4) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in rat, mouse and xenopus.

Format: Lyophilized

Description: FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in varioustransmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. TISSUE SPECIFICITY: Strongly expressed in lung and placenta, whereas it is weakly expressed in brain and heart. Also known as: Transducin beta chain 4, GNB4, Guanine nucleotide binding protein subunit beta-4.

Specificity: Appears to be specific for GBB4.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: von Weizsaecker, Strathmann, Simon: "Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA." in: Biochemical and biophysical research communications, Vol. 183, Issue 1, pp. 350-6, 1992 (PubMed).

Betty, Harnish, Rhodes et al.: "Distribution of heterotrimeric G-protein beta and gamma subunits in the rat brain." in: Neuroscience, Vol. 85, Issue 2, pp. 475-86, 1999 (PubMed).

Arnot, Stotz, Jarvis et al.: "Differential modulation of N-type 1B and P/Q-type 1A calcium channels by different G protein subunit isoforms." in: The Journal of physiology, Vol. 527 Pt 2, pp. 203-12, 2000 (PubMed).

Ruiz-Velasco, Ikeda, Puhl: "Cloning, tissue distribution, and functional expression of the human G protein beta 4-subunit." in: Physiological genomics, Vol. 8, Issue 1, pp. 41-50, 2002 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).