GBB5 (AA 50-150) antibody

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AA 50-150
Human, Rat (Rattus), Mouse (Murine)
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from aa region 50-150 of human GBB5 conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for GBB5.
Purification Whole serum
Background Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in varioustransmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Subunit: G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP.
Tissue specificity: Expressed in multiple tissues. Also known as: Guanine nucleotide-binding protein subunit beta-5, GBB5, Gbeta5, GNB5.
Application Notes A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Krispel, Chen, Melling, Chen, Martemyanov, Quillinan, Arshavsky, Wensel, Chen, Burns: "RGS expression rate-limits recovery of rod photoresponses." in: Neuron, Vol. 51, Issue 4, pp. 409-16, 2006 (PubMed).

Betty, Harnish, Rhodes, Cockett: "Distribution of heterotrimeric G-protein beta and gamma subunits in the rat brain." in: Neuroscience, Vol. 85, Issue 2, pp. 475-86, 1999 (PubMed).

Jones, Lombardi, Cockett: "Cloning and tissue distribution of the human G protein beta 5 cDNA." in: Biochimica et biophysica acta, Vol. 1402, Issue 3, pp. 288-91, 1998 (PubMed).

Watson, Aragay, Slepak, Simon: "A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina." in: The Journal of biological chemistry, Vol. 271, Issue 45, pp. 28154-60, 1996 (PubMed).

Watson, Katz, Simon: "A fifth member of the mammalian G-protein beta-subunit family. Expression in brain and activation of the beta 2 isotype of phospholipase C." in: The Journal of biological chemistry, Vol. 269, Issue 35, pp. 22150-6, 1994 (PubMed).