Guanine Nucleotide Binding Protein (G Protein), beta 5 (GNB5) (internal) antibody

Antigen: Guanine Nucleotide Binding Protein (G Protein), beta 5 (GNB5)

Synonyms: GB5, FLJ37457, FLJ43714, GBS, flr, Gbeta5, MGC105255

Epitope: internal

Clonality: Polyclonal

Host: Sheep

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350335

Additional Information

Alternative name: GBB5 (Transducin beta chain 5, Gbeta5, GNB5) (internal region)

Swiss-Prot: O14775

Immunogen: A synthetic peptide from an internal part of human, rat, mouse, rabbit GBB5 (Transducin beta chain 5, Gbeta5, GNB5) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in varioustransmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP. TISSUE SPECIFICITY: Expressed in multiple tissues. Also known as: Guanine nucleotide-binding protein subunit beta-5, GBB5, Gbeta5, GNB5.

Specificity: Appears to be specific for GBB5.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Watson, Katz, Simon: "A fifth member of the mammalian G-protein beta-subunit family. Expression in brain and activation of the beta 2 isotype of phospholipase C." in: The Journal of biological chemistry, Vol. 269, Issue 35, pp. 22150-6, 1994 (PubMed).

Watson, Aragay, Slepak et al.: "A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina." in: The Journal of biological chemistry, Vol. 271, Issue 45, pp. 28154-60, 1996 (PubMed).

Jones, Lombardi, Cockett: "Cloning and tissue distribution of the human G protein beta 5 cDNA." in: Biochimica et biophysica acta, Vol. 1402, Issue 3, pp. 288-91, 1998 (PubMed).

Betty, Harnish, Rhodes et al.: "Distribution of heterotrimeric G-protein beta and gamma subunits in the rat brain." in: Neuroscience, Vol. 85, Issue 2, pp. 475-86, 1999 (PubMed).

Krispel, Chen, Melling et al.: "RGS expression rate-limits recovery of rod photoresponses." in: Neuron, Vol. 51, Issue 4, pp. 409-16, 2006 (PubMed).