RACK1 (RACK1) (internal) antibody

Antigen: RACK1 (RACK1)

Synonyms: H12.3, HLC-7, PIG21, RACK1, Gnb2-rs1, p205, Rack1, GB-like, AL033335, rack1, wu:fb80d08, wu:fk65d12, i173, DmelCG7111, CG7111, GB11076, h12.3, hlc-7, pig21, MGC89209, gnb2-rs1, rack, dyak_GLEANR_2121, DyakGE18331, GE18331, LOC100136700, RACK, gnb2l1

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350336

Additional Information

Alternative name: GBLP (RACK1) (internal region)

Swiss-Prot: P63244

Immunogen: A synthetic peptide from the internal region of human GBLP (RACK1, GNB2L1, Receptor for activated C kinase) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in many species including rat, mouse and xenopus.

Format: Lyophilized

Description: Function: Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation. Subcellular location: Cell membrane. Note: Located on plasma membrane of neuroblastoma NMB7 cells. Also known as: Guanine nucleotide-binding protein subunit beta-like protein 12.3, Receptor of activated protein kinase C 1, RACK1, Receptor for activated C kinase, Guanine nucleotide-binding protein subunit beta-2-like 1, GNB2L1.

Specificity: Appears to be specific for GBLP.

Synonyms: H12.3, HLC-7, PIG21, RACK1, Gnb2-rs1, p205, Rack1, GB-like, AL033335, rack1, wu:fb80d08, wu:fk65d12, i173, DmelCG7111, CG7111, GB11076, h12.3, hlc-7, pig21, MGC89209, gnb2-rs1, rack, dyak_GLEANR_2121, DyakGE18331, GE18331, LOC100136700, RACK, gnb2l1

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Watson, Katz, Simon: "A fifth member of the mammalian G-protein beta-subunit family. Expression in brain and activation of the beta 2 isotype of phospholipase C." in: The Journal of biological chemistry, Vol. 269, Issue 35, pp. 22150-6, 1994 (PubMed).

Watson, Aragay, Slepak et al.: "A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina." in: The Journal of biological chemistry, Vol. 271, Issue 45, pp. 28154-60, 1996 (PubMed).

Jones, Lombardi, Cockett: "Cloning and tissue distribution of the human G protein beta 5 cDNA." in: Biochimica et biophysica acta, Vol. 1402, Issue 3, pp. 288-91, 1998 (PubMed).

Betty, Harnish, Rhodes et al.: "Distribution of heterotrimeric G-protein beta and gamma subunits in the rat brain." in: Neuroscience, Vol. 85, Issue 2, pp. 475-86, 1999 (PubMed).

Krispel, Chen, Melling et al.: "RGS expression rate-limits recovery of rod photoresponses." in: Neuron, Vol. 51, Issue 4, pp. 409-16, 2006 (PubMed).