Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1) (GAT domain) antibody

Antigen: Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1)

Epitope: GAT domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350337

Additional Information

Alternative name: GGA1 (Golgi-localized, gamma ear-containing, ARF-binding protein 1) (GAT domain)

Swiss-Prot: Q9UJY5

Immunogen: A synthetic peptide from the GAT domain of human GGA1 (Golgi-localized, gamma ear-containing, ARF-binding protein 1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many other species including rat, mouse, xenopus, zebrafish.

Format: Lyophilized

Description: FUNCTION: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. TISSUE SPECIFICITY: Ubiquitously expressed. Also known as: ADP-ribosylation factor-binding protein GGA1; Golgi-localized, gamma ear-containing, ARF-binding protein 1; Gamma-adaptin-related protein 1.

Specificity: Appears to be specific for GGA1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

DellAngelica, Puertollano, Mullins et al.: "GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 81-94, 2000 (PubMed).

Boman, Zhang, Zhu et al.: "A family of ADP-ribosylation factor effectors that can alter membrane transport through the trans-Golgi." in: Molecular biology of the cell, Vol. 11, Issue 4, pp. 1241-55, 2000 (PubMed).

He, Zhu, Koelsch et al.: "Biochemical and structural characterization of the interaction of memapsin 2 (beta-secretase) cytosolic domain with the VHS domain of GGA proteins." in: Biochemistry, Vol. 42, Issue 42, pp. 12174-80, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).