Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3) (VHS Domain) antibody

Details for Product No. ABIN350341
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms C230037M19Rik, mKIAA0154
Epitope
VHS Domain
(4), (4), (3), (3), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Human
(33), (18), (18), (12), (12), (4), (2), (2), (1), (1)
Host
Rabbit
(24), (7), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(21), (12), (10), (10), (4), (3), (2), (1)
Pubmed 5 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 7 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN350341
454.67 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide from the VHS domain of human GGA3 conjugated to an immunogenic carrier protein was used as the antigen. peptide is homologous in rat and mouse.
Specificity Specific for GGA3.
Purification Whole serum
Alternative Name GGA3
Background Function: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Subcellular location: Golgi apparatus, trans-Golgi network membrane, Peripheral membrane protein. Endosome membrane, Peripheral membrane protein.
Tissue specificity: Ubiquitously expressed. Also known as: ADP-ribosylation factor-binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, Gamma-adaptin-related protein 3.
Research Area Signaling
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3) (VHS Domain) antibody WB on rat brain lysate using Rabbit antibody to VHS domain of GGA3 (Golgi-localized, gamma ear-containing, ARF-binding protein 3): whole serum (ABIN350341) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.
General Puertollano, Aguilar, Gorshkova et al.: "Sorting of mannose 6-phosphate receptors mediated by the GGAs." in: Science (New York, N.Y.), Vol. 292, Issue 5522, pp. 1712-6, 2001 (PubMed).

Takatsu, Yoshino, Toda et al.: "GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors." in: The Biochemical journal, Vol. 365, Issue Pt 2, pp. 369-78, 2002 (PubMed).

Doray, Bruns, Ghosh et al.: "Autoinhibition of the ligand-binding site of GGA1/3 VHS domains by an internal acidic cluster-dileucine motif." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 12, pp. 8072-7, 2002 (PubMed).

Shiba, Katoh, Shiba et al.: "GAT (GGA and Tom1) domain responsible for ubiquitin binding and ubiquitination." in: The Journal of biological chemistry, Vol. 279, Issue 8, pp. 7105-11, 2004 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers. ..." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Validation Images
Did you look for something else?
back to top