Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3) (VHS Domain) antibody

Antigen: Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3)

Synonyms: KIAA0154

Binding Site: VHS Domain

Clonality: Polyclonal

Host: Sheep

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350343

Additional Information

Alternative name: GGA3 (Golgi-localized, gamma ear-containing, ARF-binding protein 3)

UniProt: Q8BMI3

Immunogen: A synthetic peptide from the VHS domain of human GGA3 (Golgi-localized, gamma ear-containing, ARF-binding protein 3) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse, chicken.

Format: Lyophilized

Description: FUNCTION: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. TISSUE SPECIFICITY: Ubiquitously expressed. Also known as: ADP-ribosylation factor-binding protein GGA3; Golgi-localized, gamma ear-containing, ARF-binding protein 3; Gamma-adaptin-related protein 3.

Specificity: Appears to be specific for GGA3.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Puertollano, Aguilar, Gorshkova et al.: "Sorting of mannose 6-phosphate receptors mediated by the GGAs." in: Science (New York, N.Y.), Vol. 292, Issue 5522, pp. 1712-6, 2001 (PubMed).

Takatsu, Yoshino, Toda et al.: "GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors." in: The Biochemical journal, Vol. 365, Issue Pt 2, pp. 369-78, 2002 (PubMed).

Doray, Bruns, Ghosh et al.: "Autoinhibition of the ligand-binding site of GGA1/3 VHS domains by an internal acidic cluster-dileucine motif." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 12, pp. 8072-7, 2002 (PubMed).

Shiba, Katoh, Shiba et al.: "GAT (GGA and Tom1) domain responsible for ubiquitin binding and ubiquitination." in: The Journal of biological chemistry, Vol. 279, Issue 8, pp. 7105-11, 2004 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).