Glutathione Peroxidase 3 (GPX3) (N-Term) antibody

Antigen: Glutathione Peroxidase 3 (GPX3)

Synonyms: GPX3, DKFZp469D1932, GPx-P, GSHPx-3, GSHPx-P, GPx, EGPx, AA960521, Gpxp, gpx3, MGC75694, GLUTATHIONE PEROXIDASE 3, T1O24.9

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350347

Additional Information

Alternative name: Glutathione peroxidase 3 (GSHPx-3, GPX3, GPXP) (n-terminal)

Swiss-Prot: P22352

Immunogen: A synthetic peptide from the n-terminal of human Glutathione peroxidase 3 (GSHPx-3, GPX3, GPXP) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in many species including rat and mouse.

Format: Lyophilized

Description: Function: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic activity: 2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O. Subcellular location: Secreted. Also known as: GPx-P, Glutathione peroxidase 3, GSHPx-3, GPX3, GPXP, GSHPx-P, GPx-3.

Specificity: Appears to be specific for GSHPx-3.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Chu, Esworthy, Doroshow et al.: "Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents." in: Blood, Vol. 79, Issue 12, pp. 3233-8, 1992 (PubMed).

Esworthy, Chu, Paxton et al.: "Characterization and partial amino acid sequence of human plasma glutathione peroxidase." in: Archives of biochemistry and biophysics, Vol. 286, Issue 2, pp. 330-6, 1991 (PubMed).

Takahashi, Akasaka, Yamamoto et al.: "Primary structure of human plasma glutathione peroxidase deduced from cDNA sequences." in: Journal of biochemistry, Vol. 108, Issue 2, pp. 145-8, 1990 (PubMed).

Yoshimura, Suemizu, Taniguchi et al.: "The human plasma glutathione peroxidase-encoding gene: organization, sequence and localization to chromosome 5q32." in: Gene, Vol. 145, Issue 2, pp. 293-7, 1994 (PubMed).

Comhair, Thomassen, Erzurum: "Differential induction of extracellular glutathione peroxidase and nitric oxide synthase 2 in airways of healthy individuals exposed to 100% O(2) or cigarette smoke." in: American journal of respiratory cell and molecular biology, Vol. 23, Issue 3, pp. 350-4, 2000 (PubMed).